New pathological condition in cultured mulloway Argyrosomus japonicus: histopathological, ultrastructural and molecular studies

D. Zilberg; J.B. Jones; M.A.A. Burger; P.K. Nicholls; D. Nolan; M. Crockford; F. Stephens

doi:10.3354/dao02495

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New pathological condition in cultured mulloway Argyrosomus japonicus: histopathological, ultrastructural and molecular studies

Journal article

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New pathological condition in cultured mulloway Argyrosomus japonicus: histopathological, ultrastructural and molecular studies

D. Zilberg, J.B. Jones, M.A.A. Burger, P.K. Nicholls, D. Nolan, M. Crockford and F. Stephens

Diseases of Aquatic Organisms, Vol.100(3), pp.219-230

2012

DOI: https://doi.org/10.3354/dao02495

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Abstract

Mulloway Argyrosomus japonicus is a native fish species in Western Australia, for which aquaculture production has recently been developed. A single cohort was stocked in a cage offshore at Geraldton, Western Australia, at a water depth of 6 m. Fish appeared healthy before stocking. Routine histological analysis was carried out from 10 mo post stocking and until completion of harvest (about 2.5 yr post stocking). No gross pathology was evident. Microscopically, however, granulomatous lesions were present in the kidneys of almost 100% of the fish examined. Enclosed in the granuloma was an aggregate of organisms, 4.2 to 5.4 μm in diameter. Kidney granulomas appeared as multi-focal aggregates. Granulomas at different stages of formation and finally fibrosing granulomas were observed. Granulomas also appeared infrequently in other organs: a few granulomas were found in the liver and spleen and a single granuloma in the heart of one fish. Transmission electron microscopy (TEM) revealed that the organism was composed of 2 cells, an outer cell enclosing an inner cell. The inner cell was surrounded by a double membrane and the outer cell by a single membrane. Cellular material, presumably of parasitic nature, surrounded the outer cell. The organism contained primitive mitochondria and abundant free ribosomes. Small subunit ribosomal DNA (SSU rDNA) sequence obtained by PCR revealed an 84% sequence identity with the myxosporean Latyspora scomberomori. Based on TEM and preliminary molecular results, we suggest that the organism is the extrasporogonic developmental stage of a myxozoan parasite, which failed to form spores in the mulloway host.

Details

Title: New pathological condition in cultured mulloway Argyrosomus japonicus: histopathological, ultrastructural and molecular studies
Authors/Creators: D. Zilberg (Author/Creator) - Ben-Gurion University of the Negev
J.B. Jones (Author/Creator)
M.A.A. Burger (Author/Creator)
P.K. Nicholls (Author/Creator)
D. Nolan (Author/Creator)
M. Crockford (Author/Creator)
F. Stephens (Author/Creator) - Western Australia
Publication Details: Diseases of Aquatic Organisms, Vol.100(3), pp.219-230
Publisher: Inter-Research Science Publishing
Identifiers: 991005541362507891
Copyright: © Inter-Research 2012
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.2 Marine Biology; 3.2.2400 Myxozoa
Web Of Science research areas: Fisheries; Veterinary Sciences
ESI research areas: Plant & Animal Science