Next Generation Sequencing uncovers within-host differences in the genetic diversity of Cryptosporidium gp60 subtypes

A. Zahedi; A.W. Gofton; F. Jian; A. Paparini; C. Oskam; A.J. Ball; I.D. Robertson; U. Ryan

doi:10.1016/j.ijpara.2017.03.003

Back

Next Generation Sequencing uncovers within-host differences in the genetic diversity of Cryptosporidium gp60 subtypes

Journal article

Open access

Peer reviewed

Next Generation Sequencing uncovers within-host differences in the genetic diversity of Cryptosporidium gp60 subtypes

A. Zahedi, A.W. Gofton, F. Jian, A. Paparini, C. Oskam, A.J. Ball, I.D. Robertson and U. Ryan

International Journal for Parasitology, Vol.47(10-11), pp.601-607

2017

DOI: https://doi.org/10.1016/j.ijpara.2017.03.003

Files and links (2)

pdf

Next-Generation-Sequencing-Cryptosporidium-gp60-subtypes.pdfDownload View

Author’s VersionCC BY-NC-ND V4.0, Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

The extent of within-host genetic diversity of parasites has implications for our understanding of the epidemiology, disease severity and evolution of parasite virulence. As with many other species, our understanding of the within-host diversity of the enteric parasite Cryptosporidium is changing. The present study compared Sanger and Next Generation Sequencing of glycoprotein 60 (gp60) amplicons from Cryptosporidium hominis (n = 11), Cryptosporidium parvum (n = 22) and Cryptosporidium cuniculus (n = 8) DNA samples from Australia and China. Sanger sequencing identified only one gp60 subtype in each DNA sample: one C. hominis subtype (IbA10G2) (n = 11), four C. parvum subtypes belonging to IIa (n = 3) and IId (n = 19) and one C. cuniculus subtype (VbA23) (n = 8). Next Generation Sequencing identified the same subtypes initially identified by Sanger sequencing, but also identified additional gp60 subtypes in C. parvum and C. cuniculus but not in C. hominis, DNA samples. The number of C. parvum and C. cuniculus subtypes identified by Next Generation Sequencing within individual DNA samples ranged from two to four, and both C. parvum IIa and IId subtype families were identified within the one host in two samples. The finding of the present study has important implications for Cryptosporidium transmission tracking as well as vaccine and drug studies.

Details

Title: Next Generation Sequencing uncovers within-host differences in the genetic diversity of Cryptosporidium gp60 subtypes
Authors/Creators: A. Zahedi (Author/Creator) - Murdoch University
A.W. Gofton (Author/Creator) - Murdoch University
F. Jian (Author/Creator) - Henan Agricultural University
A. Paparini (Author/Creator) - Murdoch University
C. Oskam (Author/Creator) - Murdoch University
A.J. Ball (Author/Creator)
I.D. Robertson (Author/Creator) - Murdoch University
U. Ryan (Author/Creator) - Murdoch University
Publication Details: International Journal for Parasitology, Vol.47(10-11), pp.601-607
Publisher: Elsevier BV
Identifiers: 991005540148007891
Copyright: © 2017 Australian Society for Parasitology.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

227 File views/ downloads

80 Record Views

43 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology
ESI research areas: Microbiology