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Next generation restoration metrics: Using soil eDNA bacterial community data to measure trajectories towards rehabilitation targets
Journal article   Peer reviewed

Next generation restoration metrics: Using soil eDNA bacterial community data to measure trajectories towards rehabilitation targets

C. Liddicoat, S.L. Krauss, A. Bissett, R.J. Borrett, L.C. Ducki, S.D. Peddle, P. Bullock, M.P. Dobrowolski, A. Grigg, M. Tibbett, …
Journal of Environmental Management, Vol.310, Article 114748
2022
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Abstract

In post-mining rehabilitation, successful mine closure planning requires specific, measurable, achievable, relevant and time-bound (SMART) completion criteria, such as returning ecological communities to match a target level of similarity to reference sites. Soil microbiota are fundamentally linked to the restoration of degraded ecosystems, helping to underpin ecological functions and plant communities. High-throughput sequencing of soil eDNA to characterise these communities offers promise to help monitor and predict ecological progress towards reference states. Here we demonstrate a novel methodology for monitoring and evaluating ecological restoration using three long-term (>25 year) case study post-mining rehabilitation soil eDNA-based bacterial community datasets. Specifically, we developed rehabilitation trajectory assessments based on similarity to reference data from restoration chronosequence datasets. Recognising that numerous alternative options for microbiota data processing have potential to influence these assessments, we comprehensively examined the influence of standard versus compositional data analyses, different ecological distance measures, sequence grouping approaches, eliminating rare taxa, and the potential for excessive spatial autocorrelation to impact on results. Our approach reduces the complexity of information that often overwhelms ecologically-relevant patterns in microbiota studies, and enables prediction of recovery time, with explicit inclusion of uncertainty in assessments. We offer a step change in the development of quantitative microbiota-based SMART metrics for measuring rehabilitation success. Our approach may also have wider applications where restorative processes facilitate the shift of microbiota towards reference states.

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UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#2 Zero Hunger
#13 Climate Action
#14 Life Below Water
#15 Life on Land

Source: InCites

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Collaboration types
Industry collaboration
Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.45 Soil Science
3.45.112 Soil Carbon Dynamics
Web Of Science research areas
Environmental Sciences
ESI research areas
Environment/Ecology
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