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Open Access CC BY-NC-ND V4.0
Journal article
Nonsevere burn induces a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response postinjury
Journal of Proteome Research, Vol.23(8), pp.2893-2907
2023
Abstract
Globally, burns are a significant cause of injury that can cause substantial acute trauma as well as lead to increased incidence of chronic comorbidity and disease. To date, research has primarily focused on the systemic response to severe injury, with little in the literature reported on the impact of nonsevere injuries (<15% total burn surface area; TBSA). To elucidate the metabolic consequences of a nonsevere burn injury, longitudinal plasma was collected from adults (n = 35) who presented at hospital with a nonsevere burn injury at admission, and at 6 week follow up. A cross-sectional baseline sample was also collected from nonburn control participants (n = 14). Samples underwent multiplatform metabolic phenotyping using 1 H nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry to quantify 112 lipoprotein and glycoprotein signatures and 852 lipid species from across 20 subclasses. Multivariate data modeling (orthogonal projections to latent structures-discriminate analysis; OPLS-DA) revealed alterations in lipoprotein and lipid metabolism when comparing the baseline control to hospital admission samples, with the phenotypic signature found to be sustained at follow up. Univariate (Mann−Whitney U) testing and OPLS-DA indicated specific increases in GlycB (p-value < 1.0e −4), low density lipoprotein-2 subfractions (variable importance in projection score; VIP > 6.83e −1) and monoacyglyceride (20:4) (p-value < 1.0e −4) and decreases in circulating anti-inflammatory high-density lipoprotein-4 subfractions (VIP > 7.75e −1), phosphatidylcholines, phosphatidylglycerols, phosphatidylinositols, and phosphatidylserines. The results indicate a persistent systemic metabolic phenotype that occurs even in cases of a nonsevere burn injury. The phenotype is indicative of an acute inflammatory profile that continues to be sustained postinjury, suggesting an impact on systems health beyond the site of injury. The phenotypes contained metabolic signatures consistent with chronic inflammatory states reported to have an elevated incidence postburn injury. Such phenotypic signatures may provide patient stratification opportunities, to identify individual responses to injury, personalize intervention strategies, and improve acute care, reducing the risk of chronic comorbidity.
Details
- Title
- Nonsevere burn induces a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response postinjury
- Authors/Creators
- Monique J Ryan - Murdoch University, Australian National Phenome CentreEdward RabyLuke Whiley - Murdoch University, Centre for Computational and Systems MedicineReika Masuda - Murdoch UniversitySamantha Lodge - Murdoch University, Centre for Computational and Systems MedicinePhilipp Nitschke - Murdoch UniversityGarth L. Maker - Murdoch University, Centre for Computational and Systems MedicineJulien Wist - Murdoch University, Centre for Computational and Systems MedicineElaine Holmes - Murdoch University, Centre for Computational and Systems MedicineFiona M Wood - Fiona Stanley HospitalJeremy Nicholson - Murdoch University, Health Futures InstituteMark W FearNicola Gray Dr - Murdoch University, Centre for Computational and Systems Medicine
- Publication Details
- Journal of Proteome Research, Vol.23(8), pp.2893-2907
- Publisher
- ACS Publications
- Identifiers
- 991005619767007891
- Copyright
- © 2023 The Authors
- Murdoch Affiliation
- Australian National Phenome Centre; Health Futures Institute; Centre for Computational and Systems Medicine
- Language
- English
- Resource Type
- Journal article
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