Journal article
Novel CMS lines in pigeonpea [Cajanus cajan (L.) Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding
The Crop Journal, Vol.5(1), pp.89-94
2017
Abstract
The availability of stable cytoplasmic male sterile (CMS or A) lines coupled with a robust restoration system (R lines) is an essential prerequisite for efficient hybrid breeding. CMS-enabled hybrid technology holds immense potential to enhance the long-stagnant productivity of pigeonpea. In the present investigation, cytoplasmic substitutions were made in the nuclear backgrounds of early-maturing pigeonpea varieties or lines. Three new CMS lines (ICPL 88039A, Pusa 992A, and DPP 3-2A) resulted from genetic crosses involving cytoplasmic donors from A2 (GT 288A) and A4 (ICPA 2089) categories. In addition to visual inspection of anthers, pollen-staining techniques and scanning electron microscopy (SEM) analysis were used to confirm pollen sterility. Further, given the relevance of the plant mitochondrial genome to CMS manifestation, 25 mitochondrion-specific DNA markers were assayed on these newly developed A lines and isogenic maintainer (B) lines. DNA polymorphism between Pusa 992A and Pusa 992B as revealed by the nad7a_del marker confirmed the successful combination of sterilizing cytoplasm (A4) and nonrestoring nuclear background (Pusa 992). Such cytoplasm-specific DNA markers are required for A2-CMS as well. Further, to assess restoration ability, potential restorers were crossed with these CMS lines, and as a consequence, promising A × R combinations exhibiting 100% pollen fertility could be identified. In parallel, we also analyzed the inheritance patterns underlying fertility restoration using ICPL 88039A-derived F2 and BC1F1 populations, and established a monogenic dominant model to explain the phenomenon of A2-CMS restoration. In summary, we report the successful development of new CMS lines and describe their effective deployment in hybrid breeding of pigeonpea.
Details
- Title
- Novel CMS lines in pigeonpea [Cajanus cajan (L.) Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding
- Authors/Creators
- A. Bohra (Author/Creator) - Indian Institute of Pulses ResearchR. Jha (Author/Creator) - Indian Institute of Pulses ResearchI.P. Singh (Author/Creator) - Indian Institute of Pulses ResearchG. Pandey (Author/Creator) - Indian Institute of Pulses ResearchS. Pareek (Author/Creator) - Indian Institute of Pulses ResearchP.S. Basu (Author/Creator) - Indian Institute of Pulses ResearchS.K. Chaturvedi (Author/Creator) - Indian Institute of Pulses ResearchN.P. Singh (Author/Creator) - Indian Institute of Pulses Research
- Publication Details
- The Crop Journal, Vol.5(1), pp.89-94
- Publisher
- Elsevier B.V.
- Identifiers
- 991005541041107891
- Copyright
- © 2016 Crop Science Society of China and Institute of Crop Science, CAAS.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1922 Alternative Oxidase
- Web Of Science research areas
- Agronomy
- Plant Sciences
- ESI research areas
- Agricultural Sciences