Journal article
Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat
Theoretical and Applied Genetics, Vol.114(3), pp.451-460
02/2007
Abstract
Low-molecular-weight glutenin subunits (LMW-GS) play an important role in bread and noodle processing quality by influencing the viscoelasticity and extensibility of dough. The objectives of this study were to characterize Glu-D3 subunit coding genes and to develop molecular markers for identifying Glu-D3 gene haplotypes. Gene specific primer sets were designed to amplify eight wheat cultivars containing Glu-D3a, b, c, d and e alleles, defined traditionally by protein electrophoretic mobility. Three novel Glu-D3 DNA sequences, designated as GluD3-4, GluD3-5 and GluD3-6, were amplified from the eight wheat cultivars. GluD3-4 showed three allelic variants or haplotypes at the DNA level in the eight cultivars, which were designated as GluD3-41, GluD3-42 and GluD3-43. Compared with GluD3-42, a single nucleotide polymorphism (SNP) was detected for GluD3-43 in the coding region, resulting in a pseudo-gene with a nonsense mutation at the 119th position of deduced peptide, and a 3-bp insertion was found in the coding region of GluD3-41, leading to a glutamine insertion at the 249th position of its deduced protein. The coding regions for GluD3-5 and GluD3-6 showed no allelic variation in the eight cultivars tested, indicating that they were relatively conservative in common wheat. Based on the 12 allelic variants of three Glu-D3 genes identified in this study and three detected previously, seven STS markers were established to amplify the corresponding gene sequences in wheat cultivars containing five Glu-D3 alleles (a, b, c, d and e). The seven primer sets M2F12/M2R12, M2F2/M2R2, M2F3/M2R3, M3F1/M3R1, M3F2/M3R2, M4F1/M4R1 and M4F3/M4R3 were specific to the allelic variants GluD3-21/22, GluD3-22, GluD3-23, GluD3-31, GluD3-32, GluD3-41 and GluD3-43, respectively, which were validated by amplifying 20 Chinese wheat cultivars containing alleles a, b, c and f based on protein electrophoretic mobility. These markers will be useful to identify the Glu-D3 gene haplotypes in wheat breeding programs.
Details
- Title
- Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat
- Authors/Creators
- X.L. Zhao (Author/Creator) - Civil Aviation AuthorityX.C. Xia (Author/Creator) - Civil Aviation AuthorityZ.H. He (Author/Creator) - International Maize and Wheat Improvement CenterZ.S. Lei (Author/Creator) - Henan Academy of Agricultural SciencesR. Appels (Author/Creator) - Murdoch UniversityY. Yang (Author/Creator) - Civil Aviation AuthorityQ.X. Sun (Author/Creator) - China Agricultural UniversityW. Ma (Author/Creator) - Murdoch University
- Publication Details
- Theoretical and Applied Genetics, Vol.114(3), pp.451-460
- Publisher
- Springer Verlag
- Identifiers
- 991005544402607891
- Copyright
- © 2006 Springer-Verlag.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences