Journal article
Novel Picornavirus detected in Wild Deer: Identification, genomic characterisation, and prevalence in Australia
Viruses, Vol.13(12), Art. 2412
2021
Abstract
The use of high-throughput sequencing has facilitated virus discovery in wild animals and helped determine their potential threat to humans and other animals. We report the complete genome sequence of a novel picornavirus identified by next-generation sequencing in faeces from Australian fallow deer. Genomic analysis revealed that this virus possesses a typical picornavirus-like genomic organisation of 7554 nt with a single open reading frame (ORF) encoding a polyprotein of 2225 amino acids. Based on the amino acid identity comparison and phylogenetic analysis of the P1, 2C, 3CD, and VP1 regions, this novel picornavirus was closely related to but distinct from known bopiviruses detected to date. This finding suggests that deer/bopivirus could belong to a novel species within the genus Bopivirus, tentatively designated as “Bopivirus C”. Epidemiological investigation of 91 deer (71 fallow, 14 sambar and 6 red deer) and 23 cattle faecal samples showed that six fallow deer and one red deer (overall prevalence 7.7%, 95% confidence interval [CI] 3.8–15.0%) tested positive, but deer/bopivirus was undetectable in sambar deer and cattle. In addition, phylogenetic and sequence analyses indicate that the same genotype is circulating in south-eastern Australia. To our knowledge, this study reports for the first time a deer-origin bopivirus and the presence of a member of genus Bopivirus in Australia. Further epidemiological and molecular studies are needed to investigate the geographic distribution and pathogenic potential of this novel Bopivirus species in other domestic and wild animal species.
Details
- Title
- Novel Picornavirus detected in Wild Deer: Identification, genomic characterisation, and prevalence in Australia
- Authors/Creators
- J.L. Huaman (Author/Creator)C. Pacioni (Author/Creator)S. Sarker (Author/Creator)M. Doyle (Author/Creator)D.M. Forsyth (Author/Creator)A. Pople (Author/Creator)T.G. Carvalho (Author/Creator)K.J. Helbig (Author/Creator)
- Publication Details
- Viruses, Vol.13(12), Art. 2412
- Publisher
- MDPI
- Identifiers
- 991005543741107891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- School of Veterinary and Life Sciences; School of Environmental and Conservation Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
32 File views/ downloads
51 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.104 Virology - General
- 1.104.901 Enterovirus Research
- Web Of Science research areas
- Virology
- ESI research areas
- Microbiology