Journal article
Novel SSR Markers for Polymorphism Detection in Pigeonpea (Cajanusspp.)
Plant Breeding, Vol.129(2), pp.142-148
2010
Abstract
With an objective to expand the repertoire of molecular markers in pigeonpea (Cajanus cajan), 36 microsatellite or simple sequence repeat (SSR) loci were isolated from a SSR-enriched genomic library. Primer pairs were designed for 23 SSR loci, of which 16 yielded amplicons of expected size. Thirteen SSR markers were polymorphic amongst 32 cultivated and eight wild pigeonpea genotypes representing six Cajanus species. These markers amplified a total of 72 alleles ranging from two to eight alleles with an average of 5.5 alleles per locus. The polymorphic information content for these markers ranged from 0.05 to 0.55 with an average of 0.32 per marker. Phenetic analysis clearly distinguished all wild species genotypes from each other and from the cultivated pigeonpea genotypes. These markers should be useful for genome mapping, trait mapping, diversity studies and assessment of gene flow between populations in pigeonpea.
Details
- Title
- Novel SSR Markers for Polymorphism Detection in Pigeonpea (Cajanusspp.)
- Authors/Creators
- R.K. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsC. Prathima (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsK.B. Saxena (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsD.A. Hoisington (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsN.K. Singh (Author/Creator) - Indian Agricultural Research InstituteR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
- Publication Details
- Plant Breeding, Vol.129(2), pp.142-148
- Publisher
- Wiley
- Identifiers
- 991005543832007891
- Copyright
- © 2009 Blackwell Verlag GmbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Agronomy
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science