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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Novel STMN2 variant linked to amyotrophic lateral sclerosis risk and clinical phenotype
Frontiers in Aging Neuroscience, Vol.13, 658226
2021
Abstract
Objective: There is a critical need to establish genetic markers that explain the complex phenotypes and pathogenicity of ALS. This study identified a polymorphism in the Stathmin-2 gene and investigated its association with sporadic ALS (sALS) disease risk, age-of onset and survival duration.
Methods: The candidate CA repeat was systematically analyzed using PCR, Sanger sequencing and high throughput capillary separation for genotyping. Stathmin-2 expression was investigated using RT-PCR in patient olfactory neurosphere-derived (ONS) cells and RNA sequencing in laser-captured spinal motor neurons.
Results: In a case-control analysis of a combined North American sALS cohort (n = 321) and population control group (n = 332), long/long CA genotypes were significantly associated with disease risk (p = 0.042), and most strongly when one allele was a 24 CA repeat (p = 0.0023). In addition, longer CA allele length was associated with earlier age-of-onset (p = 0.039), and shorter survival duration in bulbar-onset cases (p = 0.006). In an Australian longitudinal sALS cohort (n = 67), ALS functional rating scale scores were significantly lower in carriers of the long/long genotype (p = 0.034). Stathmin-2 mRNA expression was reduced in sporadic patient ONS cells. Additionally, sALS patients and controls exhibited variable expression of Stathmin-2 mRNA according to CA genotype in laser-captured spinal motor neurons.
Conclusions: We report a novel non-coding CA repeat in Stathmin-2 which is associated with sALS disease risk and has disease modifying effects. The potential value of this variant as a disease marker and tool for cohort enrichment in clinical trials warrants further investigation.
Details
- Title
- Novel STMN2 variant linked to amyotrophic lateral sclerosis risk and clinical phenotype
- Authors/Creators
- F. Theunissen (Author/Creator) - Murdoch UniversityR.S. Anderton (Author/Creator) - The University of Western AustraliaF.L. Mastaglia (Author/Creator) - The University of Western AustraliaL.L. Flynn (Author/Creator) - Murdoch UniversityS.J. Winter (Author/Creator) - The University of Notre Dame AustraliaI. James (Author/Creator) - Murdoch UniversityR. Bedlack (Author/Creator) - Duke UniversityS. Hodgetts (Author/Creator) - The University of Western AustraliaS. Fletcher (Author/Creator) - Murdoch UniversityS.D. Wilton (Author/Creator) - Murdoch UniversityN.G. Laing (Author/Creator) - Harry Perkins Institute of Medical ResearchM. MacShane (Author/Creator) - Harry Perkins Institute of Medical ResearchM. Needham (Author/Creator) - Murdoch UniversityA. Saunders (Author/Creator) - Zinfandel Pharmaceuticals, Chapel Hill, NC, United States.A. MacKay-Sim (Author/Creator) - Griffith UniversityZ. Melamed (Author/Creator) - University of California San DiegoJ. Ravits (Author/Creator) - Massachusetts General HospitalD.W. Cleveland (Author/Creator)P.A. Akkari (Author/Creator)
- Publication Details
- Frontiers in Aging Neuroscience, Vol.13, 658226
- Publisher
- Frontiers
- Identifiers
- 991005542274107891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics; Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.52 Neurodegenerative Diseases
- 1.52.765 ALS Mechanisms
- Web Of Science research areas
- Geriatrics & Gerontology
- Neurosciences
- ESI research areas
- Neuroscience & Behavior