Novel and divergent viruses associated with Australian orchid-fungus symbioses

J.W.L. Ong; H. Li; K. Sivasithamparam; K.W. Dixon; M.G.K. Jones; S.J. Wylie

doi:10.1016/j.virusres.2017.11.026

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Novel and divergent viruses associated with Australian orchid-fungus symbioses

Journal article

Open access

Peer reviewed

Novel and divergent viruses associated with Australian orchid-fungus symbioses

J.W.L. Ong, H. Li, K. Sivasithamparam, K.W. Dixon, M.G.K. Jones and S.J. Wylie

Virus Research, Vol.244, pp.276-283

2018

DOI: https://doi.org/10.1016/j.virusres.2017.11.026

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Abstract

Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis sanguinea orchids, including their fungal symbionts, over two consecutive years. A generic sequencing approach, which combined dsRNA-enrichment from plant and mycelial tissues, random amplification and high throughput shotgun sequencing was used to identify novel viruses. The majority of the virus-like sequences represent partial genomes, and their identification is based solely on de novo assembly of sequencing data. In orchid leaf tissues we found three isolates of a novel totivirus and an unclassified virus; both resemble fungus-infecting viruses. Two isolates of Ceratobasidium sp that were isolated from orchid underground stems contained at least 20 viruses, 16 of which were previously described as alphapartitiviruses and betapartitiviruses. A novel hypovirus and a mitovirus were genetically distant from existing members of the genera and did not readily fit into recognised subgroups.

Details

Title: Novel and divergent viruses associated with Australian orchid-fungus symbioses
Authors/Creators: J.W.L. Ong (Author/Creator) - Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, 6150, Australia.
H. Li (Author/Creator) - Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, 6150, Australia.
K. Sivasithamparam (Author/Creator) - Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, 6150, Australia.
K.W. Dixon (Author/Creator) - Curtin University
M.G.K. Jones (Author/Creator) - Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, 6150, Australia.
S.J. Wylie (Author/Creator) - Plant Biotechnology Group - Plant Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, 6150, Australia.
Publication Details: Virus Research, Vol.244, pp.276-283
Publisher: Elsevier BV
Identifiers: 991005542806207891
Copyright: © 2017 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.267 Virology - Plant; 3.267.356 Plant Virus Interactions
Web Of Science research areas: Virology
ESI research areas: Microbiology