Journal article
Novel genotypes of Coxiella burnetii identified in isolates from Australian Q fever patients
International Journal of Medical Microbiology, Vol.306(6), pp.463-470
2016
Abstract
Coxiella burnetii, the causative agent of Q fever, was first discovered in Australia in 1937. However, little is known about the strains of C. burnetii present in this country. In this study, six published genotyping methods were applied to 42 isolates from Australian patients with acute (n = 39) and chronic (n = 3) Q fever. All the isolates contained the plasmid QpRS and lacked the acute disease antigen A (adaA) gene. Two methods of genotyping based on single nucleotide polymorphisms (SNPs) also failed to discriminate between the isolates. However, results from the method based on SNPs within the multi-spacer sequence typing (MST) loci determined a novel MST genotype, with the Australian isolates forming a unique phylogenetic clade. Multi-locus variable number of tandem repeats (VNTR) analysis (MLVA) determined 14 genotypes, all of which were novel compared with those previously identified in strains from other countries. Many of these were single locus variants, differing from each other at just one of the 15 loci tested. Our results show that the Australian isolates exhibit significant diversity from previously characterised strains, but are genetically closely related to each other, supporting a model of evolution by clonal expansion in a geographically isolated location.
Details
- Title
- Novel genotypes of Coxiella burnetii identified in isolates from Australian Q fever patients
- Authors/Creators
- G. Vincent (Author/Creator) - Murdoch UniversityJ. Stenos (Author/Creator) - Murdoch UniversityJ. Latham (Author/Creator) - Public Health EnglandS. Fenwick (Author/Creator) - Murdoch UniversityS. Graves (Author/Creator) - Geelong Football Club
- Publication Details
- International Journal of Medical Microbiology, Vol.306(6), pp.463-470
- Publisher
- Elsevier GmbH
- Identifiers
- 991005541414807891
- Copyright
- © 2016 Elsevier GmbH
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.258 Zoonotic Diseases
- 1.258.2263 Coxiella Burnetii
- Web Of Science research areas
- Microbiology
- Virology
- ESI research areas
- Microbiology