Journal article
Novel microsatellites from the European plaice (Pleuronectes platessa) – identification by data mining and cross-species amplification in other flatfishes
Conservation Genetics, Vol.10(5), pp.1565-1568
2009
Abstract
The European plaice (Pleuronectes platessa; Pleuronectidae) is considered the most important flatfish for fisheries in Europe. Overall catches have been reduced drastically throughout the past decades, indicating a severe decline in the natural populations. However, a limited set of genetic tools are available to improve stock assessment and management for the species. Sequences of European plaice and its congener, the winter flounder (Pleuronectes americanus) deposited in the public database were searched for short tandem repeats. Out of 22 loci derived from database sequences, eight were amplified successfully and genotyping of 23 wild-caught individuals showed expected heterozygosities ranging from 0.18 to 0.84. The average allele number was 4.75 per locus (range 2–11). Successful cross-species amplification of the markers was demonstrated from two related flatfishes. All eight markers were successfully amplified from the European flounder (Platichthys flesus), a phylogenetically closely related species, whereas three of them worked in Achiroides melanorhynchus, an endemic species of Southeast Asia for which no microsatellites have been described to date.
Details
- Title
- Novel microsatellites from the European plaice (Pleuronectes platessa) – identification by data mining and cross-species amplification in other flatfishes
- Authors/Creators
- L. Casas (Author/Creator) - Universidade de Santiago de CompostelaL. Sánchez (Author/Creator) - Universidade de Santiago de CompostelaL. Orbán (Author/Creator) - National University of Singapore
- Publication Details
- Conservation Genetics, Vol.10(5), pp.1565-1568
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005544098807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.64 Phylogenetics & Genomics
- 3.64.71 Genetic Diversity
- Web Of Science research areas
- Biodiversity Conservation
- Genetics & Heredity
- ESI research areas
- Environment/Ecology