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Nutritional compounds influence tissue factor expression and inflammation of chronic kidney disease patients in vitro
Journal article   Peer reviewed

Nutritional compounds influence tissue factor expression and inflammation of chronic kidney disease patients in vitro

C.M. Shing, M.J. Adams, R.G. Fassett and J.S. Coombes
Nutrition, Vol.27(9), pp.967-972
2011
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Abstract

Objective Nutritional compounds that potentially limit inflammation and tissue factor expression may decrease the progression of chronic kidney disease (CKD) and associated cardiovascular disease. This project aimed to determine the effect of curcumin, bovine colostrum, and fish oil on inflammatory cytokine and tissue factor procoagulant activity of peripheral blood mononuclear cells (PBMCs) from patients with CKD before dialysis. Methods Peripheral blood mononuclear cells from patients with CKD before dialysis (n = 13) and age- and sex-matched healthy controls (n = 12) were cultured alone and with low and high doses of the nutritional compounds for 24 h. Cells were cultured with and without lipopolysaccharide. Supernatants were analyzed for tumor necrosis factor-α, interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1, IL-1β, C-reactive protein, and tissue factor procoagulant activity. Results The production of C-reactive protein, monocyte chemoattractant protein-1, IL-6, and IL-1β by PBMCs was inhibited by low- and high-dose fish oil in the CKD group (P < 0.05). Curcumin decreased secretion of IL-6 (P = 0.015) and IL-1 β (P = 0.016). Curcumin was more effective than colostrum at decreasing the procoagulant activity of PBMCs in the CKD and control groups (P < 0.019). Conclusion Fish oil decreased inflammatory cytokine secretion from CKD PBMCs. In addition, the beneficial effects of curcumin were demonstrated in decreasing inflammation in vitro, often to a similar magnitude as fish oil.

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Collaboration types
Domestic collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.16 Phytochemicals
3.16.1399 Curcumin
Web Of Science research areas
Nutrition & Dietetics
ESI research areas
Agricultural Sciences
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