Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

K.E. Dawood; J.A.T. Morgan; F. Busfield; M. Srivastava; T.I. Fletcher; J. Sambono; L.A. Jackson; B. Venus; A.W. Philbey; A.E. Lew-Tabor

doi:10.1016/j.ijppaw.2012.12.001

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Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

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Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

K.E. Dawood, J.A.T. Morgan, F. Busfield, M. Srivastava, T.I. Fletcher, J. Sambono, L.A. Jackson, B. Venus, A.W. Philbey and A.E. Lew-Tabor

International Journal for Parasitology: Parasites and Wildlife, Vol.2, pp.54-61

2013

DOI: https://doi.org/10.1016/j.ijppaw.2012.12.001

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Abstract

Laboratory confirmation methods are important in bovine cysticerosis diagnosis as other pathologies can result in morphologically similar lesions resulting in false identifications. We developed a probe-based real-time PCR assay to identify Taenia saginata in suspect cysts encountered at meat inspection and compared its use with the traditional method of identification, histology, as well as a published nested PCR. The assay simultaneously detects T. saginata DNA and a bovine internal control using the cytochrome c oxidase subunit 1 gene of each species and shows specificity against parasites causing lesions morphologically similar to those of T. saginata. The assay was sufficiently sensitive to detect 1 fg (Ct 35.09 ± 0.95) of target DNA using serially-diluted plasmid DNA in reactions spiked with bovine DNA as well as in all viable and caseated positive control cysts. A loss in PCR sensitivity was observed with increasing cyst degeneration as seen in other molecular methods. In comparison to histology, the assay offered greater sensitivity and accuracy with 10/19 (53%) T. saginata positives detected by real-time PCR and none by histology. When the results were compared with the reference PCR, the assay was less sensitive but offered advantages of faster turnaround times and reduced contamination risk. Estimates of the assay's repeatability and reproducibility showed the assay is highly reliable with reliability coefficients greater than 0.94.

Details

Title: Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia
Authors/Creators: K.E. Dawood (Author/Creator) - New South Wales Department of Primary Industries
J.A.T. Morgan (Author/Creator) - The University of Queensland
F. Busfield (Author/Creator) - Veterinary Medical Center
M. Srivastava (Author/Creator) - New South Wales Department of Primary Industries
T.I. Fletcher (Author/Creator) - Department of Agriculture, Fisheries and Forestry
J. Sambono (Author/Creator) - Department of Agriculture, Fisheries and Forestry
L.A. Jackson (Author/Creator) - Department of Agriculture, Fisheries and Forestry
B. Venus (Author/Creator) - Department of Agriculture, Fisheries and Forestry
A.W. Philbey (Author/Creator) - New South Wales Department of Primary Industries
A.E. Lew-Tabor (Author/Creator) - The University of Queensland
Publication Details: International Journal for Parasitology: Parasites and Wildlife, Vol.2, pp.54-61
Publisher: Elsevier Limited
Identifiers: 991005543548607891
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

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