Journal article
Observations on the loss of the cellular vestment surrounding superovulated sheep oocytes
Animal Reproduction Science, Vol.15(3-4), pp.169-175
1987
Abstract
Twenty-seven Merino and Merino × Border Leicester ewes were superovulated in the following manner 10 days after sponge insertion: Day 10 - 500 i.u. PMSG + 5 mg FSH-P; Day 11 - 300 i.u. PMSG + 4 mg FSH-P; Day 12 - 3 mg FSH-P + sponge removed. Twenty-four hours after sponge removal, 100 μg GnRH was administered. Tubal oocyte collections were performed at 18-21 (Group A), 21-25 (Group B) or 25-29 (Group C) hours after GnRH administration. Oocytes were recovered by a retrograde flush of the oviducts. Ovulation rates were 3.9 ± 1.7, 9.6 ± 1.5 and 11.9 ± 1.5 for Groups A, B and C respectively. The level of cellular vestment surrounding collected oocytes was noted. Oocytes were grouped into one of four categories: I - surrounded by an expanded cumulus/corona complex; II - surrounded by a corona only; III - devoid of all cellular vestments; IV - abnormal or degenerating oocytes. In Group A, 100% (n = 5) oocytes were Type I. In Group B (n = 44), 18.2% were Type I, 61.4% Type II, 18.2% Type III and 2.2% Type IV. In Group C (n=70), 10% were Type II and 90% were Type III. Results indicate that ovulation had begun 18-21 h after GnRH, and was mostly complete by 25 h after GnRH. There was a marked rapid cumulus loss from superovulated sheep oocytes in the oviduct - within 4-6 h, oocytes were devoid of cellular vestments. The implications of these results are discussed.
Details
- Title
- Observations on the loss of the cellular vestment surrounding superovulated sheep oocytes
- Authors/Creators
- J.G.E. Thompson (Author/Creator) - Murdoch UniversityR.G. Wales (Author/Creator) - Murdoch University
- Publication Details
- Animal Reproduction Science, Vol.15(3-4), pp.169-175
- Publisher
- Elsevier BV
- Identifiers
- 991005543979707891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- Reproductive Biology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science