One naive T cell, multiple fates in CD8+ T cell differentiation

C. Gerlach; J.W.J. van Heijst; E. Swart; D. Sie; N. Armstrong; R.M. Kerkhoven; D. Zehn; M.J. Bevan; K. Schepers; T.N.M. Schumacher

doi:10.1084/jem.20091175

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One naive T cell, multiple fates in CD8+ T cell differentiation

Journal article

Open access

Peer reviewed

One naive T cell, multiple fates in CD8+ T cell differentiation

C. Gerlach, J.W.J. van Heijst, E. Swart, D. Sie, N. Armstrong, R.M. Kerkhoven, D. Zehn, M.J. Bevan, K. Schepers and T.N.M. Schumacher

Journal of Experimental Medicine, Vol.207(6), pp.1235-1246

2010

DOI: https://doi.org/10.1084/jem.20091175

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Abstract

The mechanism by which the immune system produces effector and memory T cells is largely unclear. To allow a large-scale assessment of the development of single naive T cells into different subsets, we have developed a technology that introduces unique genetic tags (barcodes) into naive T cells. By comparing the barcodes present in antigen-specific effector and memory T cell populations in systemic and local infection models, at different anatomical sites, and for TCR–pMHC interactions of different avidities, we demonstrate that under all conditions tested, individual naive T cells yield both effector and memory CD8+ T cell progeny. This indicates that effector and memory fate decisions are not determined by the nature of the priming antigen-presenting cell or the time of T cell priming. Instead, for both low and high avidity T cells, individual naive T cells have multiple fates and can differentiate into effector and memory T cell subsets.

Details

Title: One naive T cell, multiple fates in CD8+ T cell differentiation
Authors/Creators: C. Gerlach (Author/Creator) - The Netherlands Cancer Institute
J.W.J. van Heijst (Author/Creator) - The Netherlands Cancer Institute
E. Swart (Author/Creator) - The Netherlands Cancer Institute
D. Sie (Author/Creator) - The Netherlands Cancer Institute
N. Armstrong (Author/Creator) - The Netherlands Cancer Institute
R.M. Kerkhoven (Author/Creator) - The Netherlands Cancer Institute
D. Zehn (Author/Creator) - Swiss Vaccine Research Institute
M.J. Bevan (Author/Creator) - Howard Hughes Medical Institute
K. Schepers (Author/Creator) - The Netherlands Cancer Institute
T.N.M. Schumacher (Author/Creator) - The Netherlands Cancer Institute
Publication Details: Journal of Experimental Medicine, Vol.207(6), pp.1235-1246
Publisher: The Rockefeller University Press
Identifiers: 991005540776907891
Copyright: © 2010 Gerlach et al.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.6 Immunology; 1.6.127 T Cell Regulation
Web Of Science research areas: Immunology; Medicine, Research & Experimental
ESI research areas: Immunology