Journal article
Optimisation of culture conditions for in vitro infection of tomato with the root-knot nematode Meloidogyne javanica
Australasian Plant Pathology, Vol.27(2), pp.84-89
1998
Abstract
Conditions for in vitro culture of tomato seedlings (Lycopersicon esculentum) were optimised to yield high in vitro rates of infection by the root-knot nematode Meloidogyne javanica. The influence of nutrient salts, gelling agents, sucrose concentration and pH on the development of nematode-induced root galis was investigated. A quarter-strength Murashige and Skoog medium supplemented by 0.5% sucrose and solidified with 0.6% phytagel at pH 6.4 gave most galis on tomato roots. Eggs were sterilised in 2.5% sodium hypochlorite for 4 min followed by 0.2% mercuric chloride for 4 min. Surface-sterilised eggs showed a 16% cumulative hatching rate within 10 days. Tomato seedlings cultivated in vitro for 1 week were inoculated with sterile eggs and the infection process was monitored weekly. After 5 to 7 weeks, sterile egg masses were harvested, second-stage juveniles were hatched in sterile distilled water and used to re-inoculate tomato seedlings without further sterilisation. The culture conditions described gave similar infection results for the related root-knot nematodes M. incognita, M. arenaria and M. hapla.
Details
- Title
- Optimisation of culture conditions for in vitro infection of tomato with the root-knot nematode Meloidogyne javanica
- Authors/Creators
- P. Hutangura (Author/Creator)M.G.K. Jones (Author/Creator)T. Heinrich (Author/Creator)
- Publication Details
- Australasian Plant Pathology, Vol.27(2), pp.84-89
- Publisher
- CSIRO Publishing
- Identifiers
- 991005543975307891
- Copyright
- © Australasian Plant Pathology Society 1998
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1108 Nematode Management
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science