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Journal article
Optimization of Panax notoginseng root extract hydrolysis by Cordyceps militaris derived glycosidase and bioactivities of hydrolysis products
Scientific African, Vol.15, e01082
2022
Abstract
Ginsenosides are saponins found in plants of panax genus. Despite having a wide range of health benefits, they cannot optimally reach their target tissues and organs due to their low intestinal absorption. However, reduction of their sugar moieties can produce more absorbable and bioactive compounds. Even though they are known as a good source of glycosidase, edible and medicinal mushrooms are less investigated for their capability to hydrolyse ginsenosides. An enzyme from Cordyceps militaris was used to convert ginsenosides from Panax notoginseng roots. Response surface methodology was used to study the effect of temperature (30 – 60 °C), pH (6 – 7), time (30–80 h) and enzyme concentration (0.5 – 1%), on minor ginsenoside production and optimum conditions for maximum ginsenoside hydrolysis. Regression analysis was used to develop a second-degree polynomial model for the production of minor ginsenosides. Statistical significance of the model was evidenced by coefficient of determination (R2 = 95.15%) and (P value = 0.000). Minor ginsenosides production was affected by enzyme concentration, temperature and time respectively in order of magnitude. The effect of pH was found insignificant. Maximal minor ginsenosides were found at 0.86% enzyme concentration, 42.88 °C, 62.63 h and pH 6.62. A significant difference between hydrolysates from different treatments was observed for DPPH scavenging activity and antimicrobial activity. Hydrolysates have shown a strong cytotoxic activity against (SK-LU-1) and (MCF-7) cell lines. The model developed for Panax notoginseng hydrolysis by C. militaris derived glycosidase can potentially be used for producing minor ginsenosides with improved bioactivities for use in the production of food and medicines.
Details
- Title
- Optimization of Panax notoginseng root extract hydrolysis by Cordyceps militaris derived glycosidase and bioactivities of hydrolysis products
- Authors/Creators
- Jean Baptiste Simurabiye - Nha Trang UniversityLe Thi Hoang Yen - Institute of Microbiology and Biotechnology, Vietnam National University, Ha Noi, Xuan Thuy, Cau Giay, Hanoi, 100000, VietnamDoan Duc NguyenTrinh Dac Hoanh - Vietnam Academy of Science and TechnologyVu Duy Nhan - Vietnam Academy of Science and TechnologyJean Bosco Nshimiyimana - Protestant University of RwandaTran Huyen Thanh - Institute of Microbiology and Biotechnology, Vietnam National University, Ha Noi, Xuan Thuy, Cau Giay, Hanoi, 100000, VietnamDong Thi Hoang Anh - Institute of Microbiology and Biotechnology, Vietnam National University, Ha Noi, Xuan Thuy, Cau Giay, Hanoi, 100000, Vietnam
- Publication Details
- Scientific African, Vol.15, e01082
- Publisher
- Elsevier B.V. on behalf of African Institute of Mathematical Sciences / Next Einstein Initiative.
- Number of pages
- 11
- Identifiers
- 991005743844607891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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