Journal article
Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies
Medical Oncology, Vol.39(5), Art. 81
2022
Abstract
The ectopic overexpression of transient receptor potential vanilloid-1 (TRPV1) has been detected in numerous solid cancers, including breast, prostate, pancreatic, and tongue epithelium cancer. However, the expression of TRPV1 in hematological malignancies remains unknown. Here we show through in silico analysis that elevated TRPV1 mRNA expression occurs in a range of hematological malignancies and presents an optimized flow cytometry method to rapidly assess TRPV1 protein expression for both cell lines and primary patient samples. Three anti-TRPV1 antibodies were evaluated for intracellular TRPV1 detection using flow cytometry resulting in an optimized protocol for the evaluation of TRPV1 in hematological malignant cell lines and patients’ peripheral blood mononuclear cells (PBMC). Overexpression of TRPV1 was observed in THP-1 (acute monocytic leukemia) and U266B1 (multiple myeloma, MM), but not U937 (histiocytic lymphoma) compared to healthy PBMC. TRPV1 was also detected in all 49 patients including B-cell non-Hodgkin’s lymphoma (B-NHL), MM, and others and 20 healthy controls. TRPV1 expression was increased in 8% of patients (MM = 2, B-NHL = 2). In conclusion, we provide an optimized flow cytometry method for routine expression analysis of clinical samples and show that TRPV1 is increased in a subset of patients with hematological malignancies.
Details
- Title
- Optimized flow cytometric detection of transient receptor potential vanilloid-1 (TRPV1) in human hematological malignancies
- Authors/Creators
- S.A. Omari (Author/Creator) - University of TasmaniaD.P. Geraghty (Author/Creator) - University of TasmaniaA.A. Khalafallah (Author/Creator) - University of TasmaniaP. Venkat (Author/Creator) - Cancer Institute of New South WalesY.M. Shegog (Author/Creator) - University of TasmaniaS.J. Ragg (Author/Creator) - Royal Hobart HospitalC.E. de Bock (Author/Creator) - Cancer Institute of New South WalesM.J. Adams (Author/Creator) - University of Tasmania
- Publication Details
- Medical Oncology, Vol.39(5), Art. 81
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005540416307891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- College of Science, Health, Engineering and Education
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.79 Molecular & Cell Biology - Physiology
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- Web Of Science research areas
- Oncology
- ESI research areas
- Clinical Medicine