Journal article
Oral immunization of trout fry with recombinant Lactococcus lactis NZ3900 expressing G gene of viral hemorrhagic septicaemia virus (VHSV)
Fish & Shellfish Immunology, Vol.105, pp.62-70
2020
Abstract
This study has investigated the ability of Lactococcus lactic (NZ3900) carried G gene of viral haemorrhagic septicaemia virus (VHSV) under nisin-controlled gene expression (NICE) system in rainbow trout (O.Mykiss). Two groups of trout fry (7 ± 0.65 g) were immunized with 1 × 1010 cfu/g and 1 × 108 cfu/g recombinant L. lactis NZ3900, two groups of fish were fed 1 × 1010 cfu/g and 1 × 108 cfu/g L. lactis vector free, and one group was fed by the basal diet as a control. Oral immunization was done on days 1–7 and boosting was performed on days 15–21. The relative expression of IFN-1 and MX-1 genes significantly increased in head kidney of vaccinated fish depend on vaccine dosage compared to the control group. Fish in vaccinated group also showed elevated VHSV-specific antibody levels compared to the control groups. Relative percent survival (RPS), under virulent isolate VHSV challenge were estimated 62%, 78% for 108 cfu/g 1010 cfu/g feed vaccinated groups 21 days post-vaccination, while groups fed similar doses of L. lactis vector free illustrated 22% and 27% RPSs, respectively. The significant reduction of viral loads (transcript levels of N gene) were detected in the immunized groups. Increased weight gain and decreased feed consumption in vaccinated group attributed to the probiotic effect were also observed. In conclusion, our results demonstrate the ability of recombinant L. lactis as oral vaccine against VHS in rainbow trout, which can be considered as effective method against different fish pathogens.
Details
- Title
- Oral immunization of trout fry with recombinant Lactococcus lactis NZ3900 expressing G gene of viral hemorrhagic septicaemia virus (VHSV)
- Authors/Creators
- M. Naderi-Samani (Author/Creator) - University of TehranM. Soltani (Author/Creator) - Murdoch UniversityM. Dadar (Author/Creator) - Razi Vaccine and Serum Research InstituteA. Taheri-Mirghaed (Author/Creator) - University of TehranA. Zargar (Author/Creator)S. Ahmadivand (Author/Creator) - University of TehranR. Hassanzadeh (Author/Creator) - Iranian Legal Medicine OrganizationL.M. Goudarzi (Author/Creator) - Iranian Legal Medicine Organization
- Publication Details
- Fish & Shellfish Immunology, Vol.105, pp.62-70
- Publisher
- Elsevier Ltd
- Identifiers
- 991005542540807891
- Copyright
- © 2020 Elsevier Ltd.
- Murdoch Affiliation
- Centre for Sustainable Aquatic Ecosystems; Harry Butler Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.104 Virology - General
- 1.104.1861 Fish Viral Diseases
- Web Of Science research areas
- Fisheries
- Immunology
- Marine & Freshwater Biology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science