Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma

S. Gunasekera; A. Zahedi; M. O’Dea; B. King; P. Monis; B. Thierry; J.M. Carr; U. Ryan

doi:10.3390/microorganisms8050715

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Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma

Journal article

Open access

Peer reviewed

Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma

S. Gunasekera, A. Zahedi, M. O’Dea, B. King, P. Monis, B. Thierry, J.M. Carr and U. Ryan

Microorganisms, Vol.8(5), Article 715

2020

DOI: https://doi.org/10.3390/microorganisms8050715

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Abstract

Cryptosporidium is a major cause of severe diarrhea-related disease in children in developing countries, but currently no vaccine or effective treatment exists for those who are most at risk of serious illness. This is partly due to the lack of in vitro culturing methods that are able to support the entire Cryptosporidium life cycle, which has led to research in Cryptosporidium biology lagging behind other protozoan parasites. In vivo models such as gnotobiotic piglets are complex, and standard in vitro culturing methods in transformed cell lines, such as HCT-8 cells, have not been able to fully support fertilization occurring in vitro. Additionally, the Cryptosporidium life cycle has also been reported to occur in the absence of host cells. Recently developed bioengineered intestinal models, however, have shown more promising results and are able to reproduce a whole cycle of infectivity in one model system. This review evaluates the recent advances in Cryptosporidium culturing techniques and proposes future directions for research that may build upon these successes.

Details

Title: Organoids and bioengineered intestinal models: Potential solutions to the Cryptosporidium culturing dilemma
Authors/Creators: S. Gunasekera (Author/Creator) - Murdoch University
A. Zahedi (Author/Creator) - Murdoch University
M. O’Dea (Author/Creator) - Murdoch University
B. King (Author/Creator) - Flinders University
P. Monis (Author/Creator) - South Australian Water Corporation
B. Thierry (Author/Creator) - University of South Australia
J.M. Carr (Author/Creator) - Flinders University
U. Ryan (Author/Creator) - Murdoch University
Publication Details: Microorganisms, Vol.8(5), Article 715
Publisher: MDPI
Identifiers: 991005543368707891
Murdoch Affiliation: Vector and Waterborne Pathogens Research Group; College of Science, Health, Engineering and Education; Antimicrobial Resistance and Infectious Disease Laboratory
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Microbiology
ESI research areas: Microbiology