Journal article
Oxidation of iu-14c]acetate by the sheep conceptus between days 13 and 19 of pregnancy
Reproduction, Fertility and Development, Vol.5(2), pp.201-208
1993
Abstract
Acetate metabolism by the sheep conceptus was assessed by measuring CO2 production during a 2'5-h incubation of embryos and samples of the extraembryonic membranes in HEPES-buffered media containing 1-12 mM [U-14C]acetate. The rate of oxidation of acetate by embryonic tissue showed little change between Days 13 and 15 of pregnancy but greatly decreased by Days 17 and 19. By contrast, oxidation of the substrate by the trophoblast increased substantially with development and was five times the early rate by Day 19. Oxidation of acetate by the yolk sac also increased 4-fold between Days 17 and 19. The addition of glucose to incubations of extraembryonic membranes resulted in some reduction in the oxidation of acetate by the yolk sac and allantois but had little effect on the trophoblast. At Days 13 and 15, the rate of oxidation of acetate by the embryonic disc was 6-7 times that by the trophoblast. As development progressed, this situation was reversed and by Day 19 the trophoblast metabolized more than five times the amount of acetate per ng than did the Day-19 embryo. Although acetate metabolism by yolk sac and allantois on Day 17 was low, its metabolism by the yolk sac increased to values similar to those for the trophoblast at Day 19 but its utilization by the allantoic membrane remained low. Comparison of the estimates of ATP generated from acetate by these tissue with those published for glucose demonstrates that acetate is much less effective than glucose for the provision of metabolic energy.
Details
- Title
- Oxidation of iu-14c]acetate by the sheep conceptus between days 13 and 19 of pregnancy
- Authors/Creators
- R.G. Wales (Author/Creator)E.E. Waugh (Author/Creator)
- Publication Details
- Reproduction, Fertility and Development, Vol.5(2), pp.201-208
- Publisher
- CSIRO Publishing
- Identifiers
- 991005542891707891
- Copyright
- © CSIRO 1993.
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Developmental Biology
- Reproductive Biology
- Zoology
- ESI research areas
- Plant & Animal Science