Journal article
Oxidative stress and carbon metabolism influence Aspergillus flavus transcriptome composition and secondary metabolite production
Scientific Reports, Vol.6(1), Art. 38747
2016
Abstract
Contamination of crops with aflatoxin is a serious global threat to food safety. Aflatoxin production by Aspergillus flavus is exacerbated by drought stress in the field and by oxidative stress in vitro. We examined transcriptomes of three toxigenic and three atoxigenic isolates of A. flavus in aflatoxin conducive and non-conducive media with varying levels of H2O2 to investigate the relationship of secondary metabolite production, carbon source, and oxidative stress. We found that toxigenic and atoxigenic isolates employ distinct mechanisms to remediate oxidative damage, and that carbon source affected the isolates’ expression profiles. Iron metabolism, monooxygenases, and secondary metabolism appeared to participate in isolate oxidative responses. The results suggest that aflatoxin and aflatrem biosynthesis may remediate oxidative stress by consuming excess oxygen and that kojic acid production may limit iron-mediated, non-enzymatic generation of reactive oxygen species. Together, secondary metabolite production may enhance A. flavus stress tolerance, and may be reduced by enhancing host plant tissue antioxidant capacity though genetic improvement by breeding selection.
Details
- Title
- Oxidative stress and carbon metabolism influence Aspergillus flavus transcriptome composition and secondary metabolite production
- Authors/Creators
- J.C. Fountain (Author/Creator) - University of GeorgiaP. Bajaj (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsM. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsS.N. Nayak (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsL. Yang (Author/Creator) - University of GeorgiaV. Kumar (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA.S. Jayale (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA. Chitikineni (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsW. Zhuang (Author/Creator) - Fujian Agriculture and Forestry UniversityB.T. Scully (Author/Creator) - U.S. Horticultural Research LaboratoryR.D. Lee (Author/Creator) - University of GeorgiaR.C. Kemerait (Author/Creator) - University of GeorgiaR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsB. Guo (Author/Creator) - Agricultural Research Service
- Publication Details
- Scientific Reports, Vol.6(1), Art. 38747
- Publisher
- Springer Nature
- Identifiers
- 991005542003407891
- Copyright
- © 2016 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.198 Mycotoxins
- 3.198.400 Mycotoxin Control
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- ESI research areas
- Plant & Animal Science