Journal article
PCR-sequence-specific primer typing of HLA class I and class II alleles
Methods in molecular biology, Vol.210, pp.143-171
2003
Abstract
The use of molecular typing methods for defining human leukocyte antigen (HLA) Class I and Class II alleles is now commonplace. Molecular methods offer a greater accuracy than traditional serological methods (1–5) which is reflected in many disease and transplantation studies (6–9). This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10,11) . PCR-SSP is also variably known as allele-specific PCR or amplification refractory mutation system (ARMS).
Details
- Title
- PCR-sequence-specific primer typing of HLA class I and class II alleles
- Authors/Creators
- M. Bunce (Author/Creator)
- Publication Details
- Methods in molecular biology, Vol.210, pp.143-171
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005544289207891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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