PD-1 up-regulation on CD4 + T cells promotes pulmonary fibrosis through STAT3-mediated IL-17A and TGF-β1 production

L.J. Celada; J.A. Kropski; J.D. Herazo-Maya; W. Luo; A. Creecy; A.T. Abad; O.S. Chioma; G. Lee; N.E. Hassell; G.I. Shaginurova; Y. Wang; J.E. Johnson; A. Kerrigan; W.R. Mason; R.P. Baughman; G.D. Ayers; G.R. Bernard; D.A. Culver; C.G. Montgomery; T.M. Maher; P.L. Molyneaux; I. Noth; S.E. Mutsaers; C.M. Prêle; R. Stokes Peebles; D.C. Newcomb; N. Kaminski; T.S. Blackwell; L. Van Kaer; W.P. Drake

doi:10.1126/scitranslmed.aar8356

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PD-1 up-regulation on CD4 + T cells promotes pulmonary fibrosis through STAT3-mediated IL-17A and TGF-β1 production

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PD-1 up-regulation on CD4 + T cells promotes pulmonary fibrosis through STAT3-mediated IL-17A and TGF-β1 production

L.J. Celada, J.A. Kropski, J.D. Herazo-Maya, W. Luo, A. Creecy, A.T. Abad, O.S. Chioma, G. Lee, N.E. Hassell, G.I. Shaginurova, …

Science Translational Medicine, Vol.10(460)

2018

DOI: https://doi.org/10.1126/scitranslmed.aar8356

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Abstract

Pulmonary fibrosis is a progressive inflammatory disease with high mortality and limited therapeutic options. Previous genetic and immunologic investigations suggest common intersections between idiopathic pulmonary fibrosis (IPF), sarcoidosis, and murine models of pulmonary fibrosis. To identify immune responses that precede collagen deposition, we conducted molecular, immunohistochemical, and flow cytometric analysis of human and murine specimens. Immunohistochemistry revealed programmed cell death-1 (PD-1) up-regulation on IPF lymphocytes. PD-1+CD4+ T cells with reduced proliferative capacity and increased transforming growth factor–β (TGF-β)/interleukin-17A (IL-17A) expression were detected in IPF, sarcoidosis, and bleomycin CD4+ T cells. PD-1+ T helper 17 cells are the predominant CD4+ T cell subset expressing TGF-β. Coculture of PD-1+CD4+ T cells with human lung fibroblasts induced collagen-1 production. Strikingly, ex vivo PD-1 pathway blockade resulted in reductions in TGF-β and IL-17A expression from CD4+ T cells, with concomitant declines in collagen-1 production from fibroblasts. Molecular analysis demonstrated PD-1 regulation of the transcription factor STAT3 (signal transducer and activator of transcription 3). Chemical blockade of STAT3, using the inhibitor STATTIC, inhibited collagen-1 production. Both bleomycin administration to PD-1 null mice or use of antibody against programmed cell death ligand 1 (PD-L1) demonstrated significantly reduced fibrosis compared to controls. This work identifies a critical, previously unrecognized role for PD-1+CD4+ T cells in pulmonary fibrosis, supporting the use of readily available therapeutics that directly address interstitial lung disease pathophysiology.

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Title: PD-1 up-regulation on CD4 + T cells promotes pulmonary fibrosis through STAT3-mediated IL-17A and TGF-β1 production
Authors/Creators: L.J. Celada (Author/Creator) - Vanderbilt University
J.A. Kropski (Author/Creator) - Vanderbilt University
J.D. Herazo-Maya (Author/Creator) - Yale University
W. Luo (Author/Creator) - Vanderbilt University
A. Creecy (Author/Creator) - Oklahoma Medical Research Foundation
A.T. Abad (Author/Creator) - Vanderbilt University
O.S. Chioma (Author/Creator) - Vanderbilt University
G. Lee (Author/Creator) - Vanderbilt University
N.E. Hassell (Author/Creator) - Vanderbilt University
G.I. Shaginurova (Author/Creator) - Vanderbilt University
Y. Wang (Author/Creator) - Vanderbilt University
J.E. Johnson (Author/Creator) - Vanderbilt University
A. Kerrigan (Author/Creator) - Vanderbilt University
W.R. Mason (Author/Creator) - Vanderbilt University
R.P. Baughman (Author/Creator) - University of Cincinnati
G.D. Ayers (Author/Creator) - Vanderbilt University
G.R. Bernard (Author/Creator) - Vanderbilt University
D.A. Culver (Author/Creator) - Cleveland Clinic
C.G. Montgomery (Author/Creator) - Oklahoma Medical Research Foundation
T.M. Maher (Author/Creator) - National Institute for Health Research
P.L. Molyneaux (Author/Creator) - National Institute for Health Research
I. Noth (Author/Creator) - University of Chicago
S.E. Mutsaers (Author/Creator) - The University of Western Australia
C.M. Prêle (Author/Creator) - The University of Western Australia
R. Stokes Peebles (Author/Creator)
D.C. Newcomb (Author/Creator) - Vanderbilt University
N. Kaminski (Author/Creator) - Yale University
T.S. Blackwell (Author/Creator) - Vanderbilt University
L. Van Kaer (Author/Creator) - Vanderbilt University
W.P. Drake (Author/Creator) - Vanderbilt University
Publication Details: Science Translational Medicine, Vol.10(460)
Publisher: AAAS
Identifiers: 991005540763307891
Copyright: © 2018 American Association for the Advancement of Science
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.208 Vasculitis & Autoimmune Disorders; 1.208.1262 Idiopathic Pulmonary Fibrosis
Web Of Science research areas: Cell Biology; Medicine, Research & Experimental
ESI research areas: Biology & Biochemistry