PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS

Janeth Sanabria; Julien Wist; Cesar Pulgarin

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PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS

Journal article

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PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS

Janeth Sanabria, Julien Wist and Cesar Pulgarin

Dyna (Medellín, Colombia), Vol.78(166), pp.150-157

2011

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Abstract

Engineering

Engineering, Multidisciplinary

Science & Technology

Technology

In this work, the oxidative stress was produced by the illumination of either fixed TiO2 or suspended TiO2. and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Situ Hybridisation (FISH) methods. Under certain oxidative conditions simulating incomplete treatments, viable cells that had lost their cultivability were observed to recover all of their physiological functions when transferred to fresh nutrient media. Moreover, alterations in the nutrient assimilation processes were studied by 1H-NMR during and after the photocatalytic treatment. This allowed us to shed some light on how the damage chain propagates during the treatment. This work demonstrate that the Standard Plate Count (SPC) method commonly used to estimate the cell populations during disinfection processes is ill adapted, whereas the FISH-DVC pair offers a reliable alternative.

Details

Title: PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS
Authors/Creators: Janeth Sanabria - Universidad del Valle
Julien Wist - Universidad del Valle
Cesar Pulgarin - Ecole Polytech Fed EPFL Lausanne, Inst Chem Sci & Engn, SB, GGEC, Lausanne, Switzerland
Publication Details: Dyna (Medellín, Colombia), Vol.78(166), pp.150-157
Publisher: Universidad Nacional de Colombia Sede Medellin
Number of pages: 8
Identifiers: 991005616456707891
Murdoch Affiliation: Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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Collaboration types: International collaboration
Citation topics: 2 Chemistry; 2.74 Photocatalysts; 2.74.5 TiO2 Photocatalysis
Web Of Science research areas: Engineering, Multidisciplinary
ESI research areas: Engineering