Journal article
PKB/Akt interacts with inosine-5′ monophosphate dehydrogenase through its pleckstrin homology domain
FEBS Letters, Vol.478(3), pp.253-259
2000
Abstract
The pleckstrin homology (PH) domain of the protooncogenic serine/threonine protein kinase PKB/Akt can bind phosphoinositides. A yeast- based two-hybrid system was employed which identified inosine-5' monophosphate dehydrogenase (IMPDH) type II as specifically interacting with PKB/Akts PH domain. IMPDH catalyzes the rate-limiting step of de novo guanosine-triphosphate (GTP) biosynthesis. Using purified fusion proteins, PKB/Akts PH domain and IMPDH associated in vitro and this association moderately activated IMPDH. Purified PKB/Akt also associated with IMPDH in vitro. We could specifically pull-down PKB/Akt or IMPDH from mammalian cell lysates using glutathione-S-transferase (GST)-IMPDH or GST-PH domain fusion proteins, respectively. Additionally, PKB/Akt and IMPDH could be co- immunoprecipitated from COS cell lysates and active PKB/Akt could phosphorylate IMPDH in vitro. These results implicate PKB/Akt in the regulation of GTP biosynthesis through its interaction with IMPDH, which is involved in providing the GTP pool used by signal transducing G-proteins.
Details
- Title
- PKB/Akt interacts with inosine-5′ monophosphate dehydrogenase through its pleckstrin homology domain
- Authors/Creators
- E. Ingley (Author/Creator)B.A. Hemmings (Author/Creator)
- Publication Details
- FEBS Letters, Vol.478(3), pp.253-259
- Publisher
- Wiley
- Identifiers
- 991005541640707891
- Copyright
- © 2000 Federation of European Biochemical Societies.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
24 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.25 Molecular & Cell Biology - Cancer, Autophagy & Apoptosis
- 1.25.803 PI3K/AKT/mTOR Pathway
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology
- ESI research areas
- Biology & Biochemistry