Journal article
Pathways to false positive diagnoses using molecular genetic detection methods; Phytophthora cinnamomi a case study
FEMS Microbiology Letters, Vol.364(7), fnx009
2017
Abstract
Phytophthora cinnamomi is one of the world's most invasive plant pathogens affecting ornamental plants, horticultural crops and natural ecosystems. Accurate diagnosis is very important to determine the presence or absence of this pathogen in diseased and asymptomatic plants. In previous studies, P. cinnamomi species-specific primers were designed and tested using various polymerase chain reaction (PCR) techniques including conventional PCR, nested PCR and quantitative real-time PCR. In all cases, the primers were stated to be highly specific and sensitive to P. cinnamomi. However, few of these studies tested their primers against closely related Phytophthora species (Phytophthora clade 7). In this study, we tested these purported P. cinnamomi-specific primer sets against 11 other species from clade 7 and determined their specificity; of the eight tested primer sets only three were specific to P. cinnamomi. This study demonstrated the importance of testing primers against closely related species within the same clade, and not just other species within the same genus. The findings of this study are relevant to all species-specific microbial diagnosis.
Details
- Title
- Pathways to false positive diagnoses using molecular genetic detection methods; Phytophthora cinnamomi a case study
- Authors/Creators
- M. Kunadiya (Author/Creator)D. White (Author/Creator)W.A. Dunstan (Author/Creator)G.E.St.J. Hardy (Author/Creator)V. Andjic (Author/Creator)T.I. Burgess (Author/Creator)
- Publication Details
- FEMS Microbiology Letters, Vol.364(7), fnx009
- Publisher
- Oxford Academic Press
- Identifiers
- 991005540519307891
- Copyright
- © FEMS 2017.
- Murdoch Affiliation
- Centre for Phytophthora Science and Management; School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
Metrics
99 File views/ downloads
118 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1439 Phytophthora Management
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology