Journal article
Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint
Histochemistry and Cell Biology, Vol.123(6), pp.561-571
2005
Abstract
Perlecan is a modular heparan sulphate and/or chondroitin sulphate substituted proteoglycan of basement membrane, vascular tissues and cartilage. Perlecan acts as a low affinity co-receptor for fibroblast growth factors 1, 2, 7, 9, binds connective tissue growth factor and co-ordinates chondrogenesis, endochondral ossification and vascular remodelling during skeletal development; however, relatively little is known of its distribution in these tissues during ageing and development. The aim of the present study was to immunolocalise perlecan in the articular and epiphyseal growth plate cartilages of stifle joints in 2-day to 8-year-old pedigree merino sheep. Perlecan was prominent pericellularly in the stifle joint cartilages at all age points and also present in the inter-territorial matrix of the newborn to 19-month-old cartilage specimens. Aggrecan was part pericellular, but predominantly an extracellular proteoglycan. Perlecan was a prominent component of the long bone growth plates and displayed a pericellular as well as a strong ECM distribution pattern; this may indicate a so far unrecognised role for perlecan in the mineralisation of hypertrophic cartilage. A significant age dependant decline in cell number and perlecan levels was evident in the hyaline and growth plate cartilages. The prominent pericellular distribution of perlecan observed indicates potential roles in cell-matrix communication in cartilage, consistent with growth factor signalling, cellular proliferation and tissue development.
Details
- Title
- Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint
- Authors/Creators
- J. Melrose (Author/Creator) - Royal North Shore HospitalS. Smith (Author/Creator) - Royal North Shore HospitalM. Cake (Author/Creator) - Murdoch UniversityR.A. Read (Author/Creator) - Murdoch UniversityJ. Whitelock (Author/Creator) - UNSW Sydney
- Publication Details
- Histochemistry and Cell Biology, Vol.123(6), pp.561-571
- Publisher
- Springer Verlag
- Identifiers
- 991005540491807891
- Copyright
- © Springer-Verlag 2005
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.132 Extracellular Matrix & Cell Differentiation
- 1.132.816 Glycosaminoglycans
- Web Of Science research areas
- Cell Biology
- Microscopy
- ESI research areas
- Biology & Biochemistry