Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination

Heïdi Serra; Radim Svačina; Ute Baumann; Ryan Whitford; Tim Sutton; Jan Bartoš; Pierre Sourdille

doi:10.1038/s41467-021-21127-1

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Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination

Journal article

Open access

Peer reviewed

Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination

Heïdi Serra, Radim Svačina, Ute Baumann, Ryan Whitford, Tim Sutton, Jan Bartoš and Pierre Sourdille

Nature communications, Vol.12(1), 803

2021

DOI: https://doi.org/10.1038/s41467-021-21127-1

PMID: 33547285

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Abstract

Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like behaviour, meiotic recombination between homoeologous or alien chromosomes is suppressed through the action of several loci. Here, we report positional cloning of Pairing homoeologous 2 (Ph2) and functional validation of the wheat DNA mismatch repair protein MSH7-3D as a key inhibitor of homoeologous recombination, thus solving a half-century-old question. Similar to ph2 mutant phenotype, we show that mutating MSH7-3D induces a substantial increase in homoeologous recombination (up to 5.5 fold) in wheat-wild relative hybrids, which is also associated with a reduction in homologous recombination. These data reveal a role for MSH7-3D in meiotic stabilisation of allopolyploidy and provides an opportunity to improve wheat’s genetic diversity through alien gene introgression, a major bottleneck facing crop improvement.

Details

Title: Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination
Authors/Creators: Heïdi Serra - Génétique, Diversité, Écophysiologie des Céréales
Radim Svačina - Czech Academy of Sciences, Institute of Experimental Botany
Ute Baumann - The University of Adelaide
Ryan Whitford - The University of Adelaide
Tim Sutton - The University of Adelaide
Jan Bartoš - Czech Academy of Sciences, Institute of Experimental Botany
Pierre Sourdille - Génétique, Diversité, Écophysiologie des Céréales
Publication Details: Nature communications, Vol.12(1), 803
Publisher: Nature Publishing Group UK
Number of pages: 10
Identifiers: 991005779530007891
Copyright: © The Author(s) 2021
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics