Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand

Kanokporn Yingchanakiat; Orawan Limsivilai; Supita Sunpongsri; Waree Niyomtham; Kittitat Lugsomya; Chompoonek Yurayart

doi:10.3390/jof9050590

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Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand

Journal article

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Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand

Kanokporn Yingchanakiat, Orawan Limsivilai, Supita Sunpongsri, Waree Niyomtham, Kittitat Lugsomya and Chompoonek Yurayart

Journal of fungi (Basel), Vol.9(5), Art. 590

2023

DOI: https://doi.org/10.3390/jof9050590

PMID: 37233301

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Abstract

antifungal susceptibility

cats

genotypic characterization

phenotypic characterization

Sporothrix schenckii

sporotrichosis

Sporotrichosis, an invasive fungal infection caused by Sporothrix schenckii, has emerged in Southeast Asia, affecting cats and posing a potential zoonotic risk to humans. We evaluated 38 feline sporotrichosis cases in and around Bangkok, Thailand, from 2017 to 2021. The isolates were phenotypically and genotypically characterized. The cats infected with sporotrichosis were mainly young adults, males, and domestic short hairs with uncontrolled outdoor access, and they lived in Bangkok. All isolates showed low thermotolerance and converted to the yeast phase at 35 °C. Based on the internal transcribed spacer region of rDNA sequences, our strains belonged to S. schenckii sensu stricto and clustered with clinical clade D. Based on the concatenated tree of calmodulin and beta-tubulin genes, five groups of S. schenckii were generated, and the monophyletic clade, Group II, of Thai strains was recognized. In vitro antifungal susceptibility testing demonstrated that the MIC50 of our isolates to amphotericin B, itraconazole, and posaconazole were within the limit of the species-specific epidemiological cutoff values, suggesting that the organisms were the wild type. Addressing the outbreak of feline sporotrichosis in Thailand by providing guidelines for diagnosis and effective treatment may help control the spread of disease and reduce the risk of cat-transmitted sporotrichosis to humans.

Details

Title: Phenotypic and Genotypic Characterization and Antifungal Susceptibility of Sporothrix schenckii sensu stricto Isolated from a Feline Sporotrichosis Outbreak in Bangkok, Thailand
Authors/Creators: Kanokporn Yingchanakiat - Kasetsart University
Orawan Limsivilai - Kasetsart University
Supita Sunpongsri - Kasetsart University
Waree Niyomtham - Chulalongkorn University
Kittitat Lugsomya
Chompoonek Yurayart - Kasetsart University
Publication Details: Journal of fungi (Basel), Vol.9(5), Art. 590
Publisher: MDPI
Grant note: 62-2/03; VET 61/SRF 02 / Faculty of Veterinary Medicine, Kasetsart University, Thailand
Identifiers: 991005581069707891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.148 Medical Mycology; 1.148.931 Fungal Pathogenesis
Web Of Science research areas: Microbiology; Mycology
ESI research areas: Plant & Animal Science