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Journal article
Phospholipid Phosphatase 3 (PLPP3) Induces Oxidative Stress to Accelerate Ovarian Aging in Pigs
Cells (Basel, Switzerland), Vol.13(17), 1421
2024
PMID: 39272993
Abstract
Ovarian aging results in reproductive disorders and infertility in mammals. Previous studies have reported that the ferroptosis and autophagy caused by oxidative stress may lead to ovarian aging, but the mechanisms remain unclear. In this study, we compared the morphological characteristics between the aged and young ovaries of pigs and found that the aged ovaries were larger in size and showed more corpora lutea. TUNEL assay further showed that the apoptosis level of granulosa cells (GCs) was relatively higher in the aged ovaries than those in young ovaries, as well as the expressions of autophagy-associated genes, e.g., p62, ATG7, ATG5, and BECN1, but that the expressions of oxidative stress and aging-associated genes, e.g., SOD1, SIRT1, and SIRT6, were significantly lower. Furthermore, the RNA-seq, Western blotting, and immunofluorescence suggested that phospholipid phosphatase 3 (PLPP3) protein was significantly upregulated in the aged ovaries. PLPP3 was likely to decrease the expressions of SIRT1 and SIRT6 to accelerate cellular senescence of porcine GCs, inhibit the expressions of SOD1, CAT, FSP1, FTH1, and SLC7A11 to exacerbate oxidative stress and ferroptosis, and arouse autophagy to retard the follicular development. In addition, two SNPs of PLPP3 promoter were significantly associated with the age at puberty. g.155798586 (T/T) and g.155798718 (C/C) notably facilitated the mRNA and protein level of PLPP3. In conclusion, PLPP3 might aggravate the oxidative stress of GCs to accelerate ovarian aging, and two molecular markers of PLPP3 were identified for ovarian aging in pigs. This work not only contributes to investigations on mechanisms for ovarian aging but also provides valuable molecular markers to postpone ovarian aging in populations.
Details
- Title
- Phospholipid Phosphatase 3 (PLPP3) Induces Oxidative Stress to Accelerate Ovarian Aging in Pigs
- Authors/Creators
- Hongyan Quan - South China Agricultural UniversityYixuan Guo - South China Agricultural UniversityShuo Li - South China Agricultural UniversityYao Jiang - Murdoch UniversityQingpeng Shen - South China Agricultural UniversityYingting He - South China Agricultural UniversityXiaofeng Zhou - South China Agricultural UniversityXiaolong Yuan - South China Agricultural UniversityJiaqi Li - South China Agricultural University
- Publication Details
- Cells (Basel, Switzerland), Vol.13(17), 1421
- Publisher
- MDPI
- Number of pages
- 20
- Grant note
- 2024B03J1305 / Science and Technology Program of Guangzhou Guangdong Basic and Applied Basic Research Foundation CARS-35 / Earmarked fund for China Agriculture Research System 31902131; 32072694 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Identifiers
- 991005703764707891
- Copyright
- © 2024 by the authors.
- Murdoch Affiliation
- Centre for Healthy Ageing
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.1408 Fertility Preservation
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry