Journal article
Phylogenetic study of six species of Anopheles mosquitoes in Peninsular Malaysia based on inter-transcribed spacer region 2 (ITS2) of ribosomal DNA
Parasites & Vectors, Vol.7(1), Art. 309
2014
Abstract
Background
Molecular techniques are invaluable for investigation on the biodiversity of Anopheles mosquitoes. This study aimed at investigating the spatial-genetic variations among Anopheles mosquitoes from different areas of Peninsular Malaysia, as well as deciphering evolutionary relationships of the local Anopheles mosquitoes with the mosquitoes from neighbouring countries using the anopheline ITS2 rDNA gene.
Methods
Mosquitoes were collected, identified, dissected to check infection status, and DNA extraction was performed for PCR with primers targeting the ITS2 rDNA region. Sequencing was done and phylogenetic tree was constructed to study the evolutionary relationship among Anopheles mosquitoes within Peninsular Malaysia, as well as across the Asian region.
Results
A total of 133 Anopheles mosquitoes consisting of six different species were collected from eight different locations across Peninsular Malaysia. Of these, 65 ITS2 rDNA sequences were obtained. The ITS2 rDNA amplicons of the studied species were of different sizes. One collected species, Anopheles sinensis, shows two distinct pools of population in Peninsular Malaysia, suggesting evolvement of geographic race or allopatric speciation.
Conclusion
Anopheles mosquitoes from Peninsular Malaysia show close evolutionary relationship with the Asian anophelines. Nevertheless, genetic differences due to geographical segregation can be seen. Meanwhile, some Anopheles mosquitoes in Peninsular Malaysia show vicariance, exemplified by the emergence of distinct cluster of An. sinensis population.
Details
- Title
- Phylogenetic study of six species of Anopheles mosquitoes in Peninsular Malaysia based on inter-transcribed spacer region 2 (ITS2) of ribosomal DNA
- Authors/Creators
- J-S Sum (Author/Creator) - University of MalayaW-C Lee (Author/Creator) - University of MalayaA. Amir (Author/Creator) - University of MalayaK.A. Braima (Author/Creator) - University of MalayaJ. Jeffery (Author/Creator) - University of MalayaN.M. Abdul-Aziz (Author/Creator) - University of MalayaM-Y Fong (Author/Creator) - University of MalayaY-L Lau (Author/Creator) - University of Malaya
- Publication Details
- Parasites & Vectors, Vol.7(1), Art. 309
- Publisher
- BioMed Central
- Identifiers
- 991005540163107891
- Copyright
- © 2014 Sum et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
12 File views/ downloads
37 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis
- 1.217.59 Malaria
- Web Of Science research areas
- Parasitology
- Tropical Medicine
- ESI research areas
- Microbiology