Journal article
Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat
Plant and Cell Physiology, Vol.42(2), pp.189-196
2001
Abstract
Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon. FISH analysis using a large DNA insert library of wheat identified a 67-kb clone (R11H) that showed strong hybridization signals on the centromeres. The R11H clone contains Ty3/gypsy retrotransposon-related sequences; both integrase and CCS1 family sequences were identified. Subsequently, we isolated additional 23 large-insert clones which also contained the integrase and CCS1 sequences. Based on the number of the integrase repeats in the clones determined by DNA gel blot analysis, we concluded that the retrotransposon-like sequences are tandemly repeated in wheat centromeres in ca. 55-kb interval on average. This conclusion is consistent with the results of FISH analysis on the extended DNA fibers.
Details
- Title
- Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat
- Authors/Creators
- K-N Fukui (Author/Creator)G. Suzuki (Author/Creator)E.S. Lagudah (Author/Creator)S. Rahman (Author/Creator)R. Appels (Author/Creator)M. Yamamoto (Author/Creator)Y. Mukai (Author/Creator)
- Publication Details
- Plant and Cell Physiology, Vol.42(2), pp.189-196
- Publisher
- Oxford University Press
- Identifiers
- 991005544686307891
- Copyright
- 2001 JSPP
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Cell Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science