Journal article
Physical identification of a virus in a crude leaf extract by its ferguson plot in agarose gel electrophoresis
Electrophoresis, Vol.9(4), pp.162-166
1988
Abstract
Crude extracts of turnip crinkle virus upon agarose gel electrophoresis yield (i) virus patterns unperturbed by contaminants; (ii) plots of mobility vs. gel concentration (Ferguson plots) parallel with those of the purified virus. The parallelism suggests similarity in size and shape but a lower net charge for the crude virus. This result is obtained when gel electrophoresis is carried out either in a continuous buffer or in a discontinuous (moving boundary electrophoresis) buffer system. The latter mode has the substantial benefit of electrophoretic (auto-)concentration of dilute virus sample prior to resolution. Thus, the Ferguson plot analysis in a discontinuous buffer system of turnip crinkle virus can be viewed as a model procedure for the physical identification of other viruses contained in dilute extracts, feasible even in the absence of a prior knowledge as to the nature of, or isolation of, the virus.
Details
- Title
- Physical identification of a virus in a crude leaf extract by its ferguson plot in agarose gel electrophoresis
- Authors/Creators
- L. Orbán (Author/Creator) - Health and Human Development (2HD) Research NetworkA. Chrambach (Author/Creator) - Health and Human Development (2HD) Research Network
- Publication Details
- Electrophoresis, Vol.9(4), pp.162-166
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005543539707891
- Copyright
- © 1988 VCH Verlagsgesellschaft mbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
21 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry