Journal article
Physical measurements of the liver glucocorticoid receptor
Biochemical Journal, Vol.169(2), pp.445-448
1978
Abstract
Physical measurements were made on the cytosolic form of the liver [3H]dexamethasone receptor. These include a Stokes radius of 3.5 nm, determined by gel filtration, and sedimentation coefficients of 5.1 and 7-8S, by sucrose-density-gradient centrifugation. From these measurements, the following physical properties were calculated: apparent mol. wt. 78000 (the 5.1 S form); D app. 6.1 × 10(-7) cm2-S-1; f/fo 1.25; axial ratio 4.7; these indicate a globular protein. Measurements of sedimentation coefficient of cytosol steroid-receptor complexes previously subjected to various activating conditions gave different values and lead to the conclusion that the mechanism of activation in vitro enabling the steroid-receptor complex to bind to DNA is more complex than simple disaggregation to a uniform size.
Details
- Title
- Physical measurements of the liver glucocorticoid receptor
- Authors/Creators
- G. Litwack (Author/Creator)M.H. Cake (Author/Creator)R. Filler (Author/Creator)K. Taylor (Author/Creator)
- Publication Details
- Biochemical Journal, Vol.169(2), pp.445-448
- Publisher
- Portland Press, Ltd.
- Identifiers
- 991005543950807891
- Copyright
- © 1978 Portland Press
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
Metrics
30 Record Views