Journal article
Polymerase directed incorporation studies of LNA-G nucleoside 5′-triphosphate and primer extension involving all four LNA nucleotides
New Journal of Chemistry, Vol.34(5)
2010
Abstract
For the first time, the enzymatic incorporation of LNA-G nucleotides into DNA strands has been investigated. KOD DNA polymerase accepted LNA-GTP as a substrate and provided a full-length dsDNA product modified with LNA-G nucleotides. Most importantly, an extension experiment was conducted using a reaction mixture containing LNA-A, LNA-T, LNA-G and LNA-mC nucleotides as the only NTP sources and this showed that KOD DNA polymerase can even incorporate 21 LNA nucleotides successively to a full-length extension product. Also, the experiments revealed that LNA-containing extension products were much more resistant towards the 3′→5′ exonucleolytic cleavage activity of the enzyme than DNA extension products.
Details
- Title
- Polymerase directed incorporation studies of LNA-G nucleoside 5′-triphosphate and primer extension involving all four LNA nucleotides
- Authors/Creators
- R.N. Veedu (Author/Creator) - University of Southern DenmarkB. Vester (Author/Creator) - University of Southern DenmarkJ. Wengel (Author/Creator) - University of Southern Denmark
- Publication Details
- New Journal of Chemistry, Vol.34(5)
- Publisher
- Royal Society of Chemistry
- Identifiers
- 991005542828507891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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