Polymerase-directed synthesis of C5-ethynyl locked nucleic acids

R.N. Veedu; H.V. Burri; P. Kumar; P.K. Sharma; P.J. Hrdlicka; B. Vester; J. Wengel

doi:10.1016/j.bmcl.2010.09.044

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Polymerase-directed synthesis of C5-ethynyl locked nucleic acids

Journal article

Peer reviewed

Polymerase-directed synthesis of C5-ethynyl locked nucleic acids

R.N. Veedu, H.V. Burri, P. Kumar, P.K. Sharma, P.J. Hrdlicka, B. Vester and J. Wengel

Bioorganic & Medicinal Chemistry Letters, Vol.20(22), pp.6565-6568

2010

DOI: https://doi.org/10.1016/j.bmcl.2010.09.044

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Abstract

Modified nucleic acids have considerable potential in nanobiotechnology for the development of nanomedicines and new materials. Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far and we herein for the first time report the enzymatic incorporation of LNA-U and C5-ethynyl LNA-U nucleotides into oligonucleotides. Phusion High Fidelity and KOD DNA polymerases efficiently incorporated LNA-U and C5-ethynyl LNA-U nucleotides into a DNA strand and T7 RNA polymerase successfully accepted the LNA-U nucleoside 5′-triphosphate as substrate for RNA transcripts.

Details

Title: Polymerase-directed synthesis of C5-ethynyl locked nucleic acids
Authors/Creators: R.N. Veedu (Author/Creator) - University of Southern Denmark
H.V. Burri (Author/Creator) - University of Southern Denmark
P. Kumar (Author/Creator) - University of Idaho
P.K. Sharma (Author/Creator) - Kurukshetra University
P.J. Hrdlicka (Author/Creator) - University of Idaho
B. Vester (Author/Creator) - University of Southern Denmark
J. Wengel (Author/Creator) - University of Southern Denmark
Publication Details: Bioorganic & Medicinal Chemistry Letters, Vol.20(22), pp.6565-6568
Publisher: Elsevier BV
Identifiers: 991005542677107891
Copyright: © 2010 Elsevier Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.170 Nucleic Acids Chemistry; 2.170.988 Oligonucleotide Modifications
Web Of Science research areas: Chemistry, Medicinal; Chemistry, Organic
ESI research areas: Chemistry