Journal article
Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea
International Journal of Systematic Bacteriology, Vol.48(3), pp.659-668
1998
Abstract
The population genetics of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands province of Papua New Guinea were investigated. Multilocus enzyme electrophoresis (MLEE) was used to analyse 164 isolates from humans and animals. These were divided into 33 electrophoretic types (ETs), four of which contained 65% of the isolates. The mean genetic diversity (n = number of ETs) for 145 human isolates was 0.18, and the mean number of alleles at five polymorphic loci was 2.6. The species appeared to be recombinant, as there was a lack of linkage disequilibrium, and 25% of all the possible combinations of alleles was present in the population. PFGE analysis using the enzymes MluI and Sall divided 157 of the isolates into 99 PFGE types, demonstrating the existence of considerable strain diversity in a geographically restricted area. The two techniques were in excellent agreement; however, PFGE was more discriminatory for strain typing than was MLEE. Nine out of 19 (474%) culture-positive individuals were colonized by the same PFGE type of S. pilosicoli when retested after 6 weeks. For three individuals, the PFGE profiles of the second isolate differed from the first in only one or two DNA bands, while the other seven individuals were colonized with distinct PFGE types on each occasion. In two cases, strains with the same PFGE pattern were isolated from humans and dogs, suggesting that cross- species transmission of S. pilosicoli may occur naturally and that the infection can be zoonotic.
Details
- Title
- Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea
- Authors/Creators
- D.J. Trott (Author/Creator) - Murdoch UniversityA.S.J. Mikosza (Author/Creator)B.G. Combs (Author/Creator)S.L. Oxberry (Author/Creator)D.J. Hampson (Author/Creator)
- Publication Details
- International Journal of Systematic Bacteriology, Vol.48(3), pp.659-668
- Publisher
- Society for General Microbiology
- Identifiers
- 991005541573307891
- Copyright
- © 1998 IUMS
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
31 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2445 Lawsonia Intracellularis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology