Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)

Dandan Qin; Gang Liu; Rui Liu; Chunchao Wang; Fuchao Xu; Qing Xu; Yu Ling; Guoqing Dong; Yanchun Peng; Shuangtao Ge; Ganggang Guo; Jing Dong; Chengdao Li

doi:10.1007/s00122-023-04272-7

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Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)

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Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)

Dandan Qin, Gang Liu, Rui Liu, Chunchao Wang, Fuchao Xu, Qing Xu, Yu Ling, Guoqing Dong, Yanchun Peng, Shuangtao Ge, …

Theoretical and applied genetics, Vol.136(1), pp.7-18

2023

DOI: https://doi.org/10.1007/s00122-023-04272-7

PMID: 36656367

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Abstract

Chlorophyll

Cloning, Molecular

Edible Grain - genetics

Hordeum

Phenotype

Quantitative Trait Loci

Map-based cloning, subcellular localization, virus-induced-gene-silencing and transcriptomic analysis reveal HvTUB8 as a candidate gene with pleiotropic effects on barley spike and leaf development via ethylene and chlorophyll metabolism. Barley lateral spikelet morphology and grain shape play key roles in grain physical quality and yield. Several genes and QTLs for these traits have been cloned or fine mapped previously. Here, we report the phenotypic and genotypic analysis of a barley mutant with round lateral spikelet (rls) from cv. Edamai 934. rls had round lateral spikelet, short but round grain, shortened awn, thick glume and dark green leaves. Histocytologic and ultrastructural analysis revealed that the difference of grain shape of rls was caused by change of cell arrangement in glume, and the dark leaf color resulted from enlarged chloroplast. HvTUBULIN8 (HvTUB8) was identified as the candidate gene for rls by combination of RNA-Seq, map-based-cloning, virus-induced-gene-silencing (VIGS) and protein subcellular location. A single G-A substitution at the third exon of HvTUB8 resulted in change of Cysteine 354 to tyrosine. Furthermore, the mutant isoform Hvtub8 could be detected in both nucleus and cytoplasm, whereas the wild-type protein was only in cytoplasm and granular organelles of wheat protoplasts. Being consistent with the rare phenotype, the "A" allele of HvTUB8 was only detected in rls, but not in a worldwide barley germplasm panel with 400 accessions. VIGS confirmed that HvTUB8 was essential to maintain spike integrity. RNA-Seq results suggested that HvTUB8 may control spike morphogenesis via ethylene homeostasis and signaling, and control leaf color through chlorophyll metabolism. Collectively, our results support HvTUB8 as a candidate gene for barley spike and leaf morphology and provide insight of a novel mechanism of it in barley development.

Details

Title: Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)
Authors/Creators: Dandan Qin - Ministry of Agriculture and Rural Affairs
Gang Liu - Ministry of Agriculture and Rural Affairs
Rui Liu - Wuhan Polytechnic University
Chunchao Wang - Institute of Crop Science
Fuchao Xu - Ministry of Agriculture and Rural Affairs
Qing Xu - Ministry of Agriculture and Rural Affairs
Yu Ling - Guangdong Ocean University
Guoqing Dong - Wuhan Polytechnic University
Yanchun Peng - Ministry of Agriculture and Rural Affairs
Shuangtao Ge - Ministry of Agriculture and Rural Affairs
Ganggang Guo - Institute of Crop Science
Jing Dong - Ministry of Agriculture and Rural Affairs
Chengdao Li - Western Crop Genetics Alliance, College of Science, Health, Engineering and Education, Murdoch University, Western Australia, WA, 6150, Australia. C.Li@murdoch.edu.au
Publication Details: Theoretical and applied genetics, Vol.136(1), pp.7-18
Grant note: 2018YFD1000700 / Key Technologies Research and Development Program 31771774 / Innovative Research Group Project of the National Natural Science Foundation of China 201808420082 / China Scholarship Council 2018YFD1000706 / Key Technologies Research and Development Program CARS-05 / Agriculture Research System of China
Identifiers: 991005569352807891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Agricultural Sciences