Abstract
The suspension growth, protoplast isolation and culture of Lolium mulliflorum Lam and L.perenne-L were studied Ryegrass suspension cultures were in higher growth volume subcultured in the volume ratio of 1 ml cells to 20 ml liquid medium.The enzyme solution produccd maximum yield of the protoplasts when the proportion of cells in enzyme solution was 20%～30%. Glucose was more suitable than sucrose and mannitol in protoplast medium as carbon source or osmoticum and led to higher plating efficiency of ryegrass protoplasts.