Journal article
Prevalence and genotyping identification of Cryptosporidium in adult ruminants in central Iran
Parasites & Vectors, Vol.12, Article number: 510
2019
Abstract
Background
Apicomplexan parasites of the genus Cryptosporidium infect a wide range of animal species as well as humans. Cryptosporidium spp. can cause life threatening diarrhea especially in young animals, children, immunocompromised patients and malnourished individuals. Asymptomatic cryptosporidial infections in animals can also occur, making these animals potential reservoirs of infection.
Methods
In the present study, a molecular survey of Cryptosporidium spp. in ruminants that were slaughtered for human consumption in Yazd Province, located in central Iran was conducted. Faeces were collected per-rectum from 484 animals including 192 cattle, 192 sheep and 100 goats. DNA was extracted from all samples and screened for Cryptosporidium by PCR amplification of the 18S rRNA gene. Positives were Sanger sequenced and further subtyped by sequence analysis of the 60 kDa glycoprotein (gp60) locus.
Results
In total, Cryptosporidium spp. were detected in 22 animals: C. andersoni and C. bovis in seven and two cattle faecal samples, respectively, C. ubiquitum in five sheep, and C. xiaoi in six sheep and two goat samples, respectively. To our knowledge, this study provides for the first time, molecular information concerning Cryptosporidium species infecting goats in Iran, and is also the first report of C. ubiquitum and C. xiaoi from ruminants in Iran.
Conclusion
The presence of potentially zoonotic species of Cryptosporidium in ruminants in this region may suggest that livestock could potentially contribute to human cryptosporidiosis, in particular among farmers and slaughterhouse workers, in the area. Further molecular studies on local human populations are required to more accurately understand the epidemiology and transmission dynamics of Cryptosporidium spp. in this region.
Details
- Title
- Prevalence and genotyping identification of Cryptosporidium in adult ruminants in central Iran
- Authors/Creators
- Z. Firoozi (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesA. Sazmand (Author/Creator) - Bu-Ali Sina UniversityA. Zahedi (Author/Creator) - Murdoch UniversityA. Astani (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesA. Fattahi-Bafghi (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesN. Kiani-Salmi (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesB. Ebrahimi (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesA. Dehghani-Tafti (Author/Creator) - Shahid Sadoughi University of Medical Sciences and Health ServicesU. Ryan (Author/Creator) - Murdoch UniversityF. Akrami-Mohajeri (Author/Creator)
- Publication Details
- Parasites & Vectors, Vol.12, Article number: 510
- Publisher
- BioMed Central
- Identifiers
- 991005542034107891
- Copyright
- © The Author(s) 2019.
- Murdoch Affiliation
- College of Science, Health, Engineering and Education
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Parasitology
- Tropical Medicine
- ESI research areas
- Microbiology