Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

R. Yang; C. Jacobson; C. Gordon; U. Ryan

doi:10.1016/j.vetpar.2008.12.021

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Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Journal article

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Peer reviewed

Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

R. Yang, C. Jacobson, C. Gordon and U. Ryan

Veterinary Parasitology, Vol.161(1-2), pp.19-24

2009

DOI: https://doi.org/10.1016/j.vetpar.2008.12.021

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Abstract

A total of 477 faecal samples from pre-weaned sheep from 5 different farms in the south west of Western Australia were screened for the presence of Cryptosporidium and Giardia using PCR. There were substantial differences in prevalence between the farms and overall prevalence was 24.5% and 11.1%, respectively for Cryptosporidium and Giardia. At the 18S locus, 66 Cryptosporidium positives were identified, the majority of which were C. bovis (n = 52), followed by the cervid genotype (n = 10) and C. parvum (n = 2). At a second diagnostic locus, using C. parvum and C. hominis-specific qPCR primers, 63 C. parvum positives were identified, some of which were co-infections with C. bovis. The C. parvum/C. hominis qPCR was more sensitive than the nested 18S PCR at detecting C. parvum. This may be due to the low numbers of oocysts present, as quantitation data indicated that all the C. parvum detected were present in low numbers (1-10 oocysts). It may also be that using C. parvum-specific primers is necessary to determine the true prevalence of C. parvum. Amongst Giardia positive isolates, G. duodenalis genotype E (livestock) was the most prevalent (36/53), with G. duodenalis genotype A detected in five positive isolates. There were also 11 mixed A and E infections detected. The findings of the present study indicate that pre-weaned lambs are not an important source of zoonotic Giardia genotypes in Australia but may be an important source of zoonotic Cryptosporidium.

Details

Title: Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia
Authors/Creators: R. Yang (Author/Creator) - Murdoch University
C. Jacobson (Author/Creator) - Murdoch University
C. Gordon (Author/Creator) - Water Corporation of Western Australia (Australia)
U. Ryan (Author/Creator) - Murdoch University
Publication Details: Veterinary Parasitology, Vol.161(1-2), pp.19-24
Publisher: Elsevier BV
Identifiers: 991005540524307891
Copyright: © 2009 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology; Veterinary Sciences
ESI research areas: Plant & Animal Science