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Journal article
Proanthocyanidins isolated from the leaves of Ficus glomerata evaluated on the activities of rumen enzymes: in vitro and in silico studies
Frontiers in chemistry, Vol.12, 1359049
2024
PMID: 38380397
Abstract
Two new proanthocyanidins (2S:3S)-(−)-epicatechin-(4α→8)4-(2R:3R)-(+)-catechin (Compound 1) and (2R, 3R)-3-O-galloyl-(+)-catechin (4β→8)3-(2R, 3R)-3-O-galloyl-(+)-catechin (Compound 2) were isolated from Ficus glomerata and characterized by ultraviolet spectroscopy (UV), proton nuclear magnetic resonance (1H NMR), 13C NMR, and heteronuclear multiple bond correlation . The bioactivity and drug scores of isolated compounds were predicted using OSIRIS property explorer applications with drug scores of 0.03 (compound 1) and 0.05 (compound 2). Predictive drug scores provided an indication of the compounds’ potential to demonstrate desired biological effects. Furthermore, the newly discovered proanthocyanidins tended to interact with protein due to their chemical structure and molecular conformation. With the aim of maintaining this focus, compounds 1 and 2 were subjected to in vitro testing against ruminal enzymes to further explore their potential impact. Both compounds showed significant inhibition activities (p < 0.01) against glutamic oxaloacetic transaminase in both protozoa and bacterial fractions, with an effective concentration (EC50) of 12.30–18.20 mg/mL. The compounds also exhibited significant inhibition (p < 0.01) of ruminal glutamic pyruvic transaminase activity, with EC50 values ranging from 9.77 to 17.38 mg/mL. Furthermore, the inhibition was recorded in R-cellulase between EC50 values of 15.85 and 23.99 mg/mL by both compounds. Additionally, both compounds led to a decrease in protease activity with increasing incubation time and concentration. In conclusion, the results indicate that these novel proanthocyanidins hold the potential to significantly impact rumen enzyme biology. Furthermore, their promising effects suggest that they could be further explored for drug development and other important applications.
Details
- Title
- Proanthocyanidins isolated from the leaves of Ficus glomerata evaluated on the activities of rumen enzymes: in vitro and in silico studies
- Authors/Creators
- Suman Lata - Indian Grassland and Fodder Research InstitutePushpendra Koli - Murdoch UniversitySultan Singh - Indian Grassland and Fodder Research InstituteBrijesh Kumar Bhadoria - Indian Grassland and Fodder Research InstituteUmesh Chand - Central University of PunjabDinesh Kumar Yadav - , , , , , , , , , , , , , ,Thamer Al-Shuwaili - University of KerbalaYonglin Ren - Murdoch University
- Publication Details
- Frontiers in chemistry, Vol.12, 1359049
- Publisher
- Frontiers Media S.A.
- Identifiers
- 991005638867907891
- Copyright
- © 2024 Lata et al.
- Murdoch Affiliation
- College of Environmental and Life Sciences; Centre for Biosecurity and One Health
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.84 Ruminant Nutrition
- Web Of Science research areas
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry