Probing for pH-regulated senes in Sinorhizobium medicae using transcriptional analysis

R.P. Tiwari; W.G. Reeve; B.J. Fenner; M.J. Dilworth; A.R. Glenn; J.G. Howieson

doi:10.1159/000078656

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Probing for pH-regulated senes in Sinorhizobium medicae using transcriptional analysis

Journal article

Peer reviewed

Probing for pH-regulated senes in Sinorhizobium medicae using transcriptional analysis

R.P. Tiwari, W.G. Reeve, B.J. Fenner, M.J. Dilworth, A.R. Glenn and J.G. Howieson

Journal of Molecular Microbiology and Biotechnology, Vol.7(3), pp.133-139

2004

DOI: https://doi.org/10.1159/000078656

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Abstract

The low pH sensitivity of Sinorhizobium species is one of the major causes of reduced productivity of Medicago species (such as lucerne) sown in acidic soils. To investigate the pH response of an acid-tolerant Sinorhizobium medicae strain, a pool of random promoter fusions to gusA was created using minitransposon insertional mutagenesis. Acid-activated expression was identified in 11 mutants; rhizobial DNA flanking insertions in 10 mutants could be cloned and the DNA sequences obtained were used to interrogate the genome database of Sinorhizobium meliloti strain 1021. Acid activated expression was detected for fixNO, kdpC, lpiA, and phrR and for genes encoding a putative lipoprotein, two ABC-transporter components, a putative DNA ligase and a MPA1-family protein. These findings implicate cytochrome synthesis, potassium ion cycling, lipid biosynthesis and transport processes as key components of pH response in S. medicae.

Details

Title: Probing for pH-regulated senes in Sinorhizobium medicae using transcriptional analysis
Authors/Creators: R.P. Tiwari (Author/Creator) - Murdoch University
W.G. Reeve (Author/Creator)
B.J. Fenner (Author/Creator)
M.J. Dilworth (Author/Creator)
A.R. Glenn (Author/Creator)
J.G. Howieson (Author/Creator)
Publication Details: Journal of Molecular Microbiology and Biotechnology, Vol.7(3), pp.133-139
Publisher: S. Karger AG
Identifiers: 991005542754307891
Copyright: © 2004 S. Karger AG, Basel
Murdoch Affiliation: Centre for Rhizobium Studies
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.892 Rhizobium-Legume Symbiosis
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Microbiology