Journal article
Production and characterization of a panel of Monoclonal antibodies against native human cellular prion protein
Hybridoma, Vol.28(1), pp.13-20
2009
Abstract
The human prion diseases, such as variant Creutzfeldt-Jakob disease (vCJD), are characterized by the conversion of the normal cellular prion protein (PrPC) into an abnormal disease associated form (PrPSc). Monoclonal antibodies (MAbs) that recognize these different PrP isoforms are valuable reagents both in the diagnosis of these diseases and in prion disease research in general but we know of no attempts to raise MAbs against native human PrPC. We immunized prion protein gene ablated (PrP-/-) mice with native human PrPC purified from platelets (pHuPrP) generating a predominantly IgG isotype anti-pHuPrP polyclonal antibody response in all mice. Following fusion of splenocytes from the immunized mice with SP2/0 myeloma cells, we were able to identify single cell clone and cryopreserve 14 stable hybridoma cell lines producing MAbs that reacted with pHuPrP. The properties of these MAbs (such as isotype, binding to native/denatured pHuPrP, and HuPrP epitopes recognized) are described. Furthermore, several of these MAbs showed a selectivity in their ability to immunoprecipitate disease associated PrPSc and its corresponding protease resistant core (PrPres).
Details
- Title
- Production and characterization of a panel of Monoclonal antibodies against native human cellular prion protein
- Authors/Creators
- M. Jones (Author/Creator) - Western General HospitalV. McLoughlin (Author/Creator) - Strathclyde Institute of Pharmacy and Biomedical SciencesJ.G. Connolly (Author/Creator)C.F. Farquhar (Author/Creator)I.R. MacGregor (Author/Creator)M.W. Head (Author/Creator) - Deanery of Molecular, Genetic and Population Health Sciences
- Publication Details
- Hybridoma, Vol.28(1), pp.13-20
- Publisher
- Mary Ann Liebert
- Identifiers
- 991005543504207891
- Copyright
- © 2009, Mary Ann Liebert, Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
17 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.52 Neurodegenerative Diseases
- 1.52.992 Prion Pathogenesis
- Web Of Science research areas
- Biochemical Research Methods
- Biotechnology & Applied Microbiology
- Immunology
- ESI research areas
- Immunology