Journal article
Production of somatic hybrids by electrofusion in Solanum
Theoretical and Applied Genetics, Vol.76(2), pp.260-266
1988
Abstract
Conditions are described for large scale electrofusion of mesophyll protoplasts of dihaploid S. tuberosum with those of diploid S. brevidens. Overall fusion frequencies of 20%–30% were achieved, and following fusion, large numbers of protoplast-derived calli were obtained. Putative somatic hybrid plants were selected from the regenerated shoots by examining their morphological characteristics. Twenty-one somatic hybrids were confirmed by isoenzyme analysis and six somatic hybrids were further confirmed by Southern hybridization. Tetraploid hybrids were obtained, but cytogenetic studies indicated that more of the regenerated hybrids were hexaploid than had previously been found following chemical fusion of the same partners. Some advantages of electrofusion over chemical fusion are discussed.
Details
- Title
- Production of somatic hybrids by electrofusion in Solanum
- Authors/Creators
- N. Fish (Author/Creator)A. Karp (Author/Creator)M.G.K. Jones (Author/Creator)
- Publication Details
- Theoretical and Applied Genetics, Vol.76(2), pp.260-266
- Publisher
- Springer Verlag
- Identifiers
- 991005544639907891
- Copyright
- © 1988 Springer-Verlag
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences