Journal article
Progression of anemia and its relationship with renal function, blood pressure, and erythropoietin in rats with chronic kidney disease
Veterinary Clinical Pathology, Vol.44(3), pp.342-354
2015
Abstract
Background: In chronic kidney disease (CKD), anemia and hypertension are significant co-morbidities that contribute to cardiovascular and renal disease progression. Objective: The purpose of the study was to identify correlations between changes in hematologic variables against markers of renal function, blood pressure, and erythropoietin (EPO) in a naturally occurring hypertensive model of CKD, the Lewis polycystic kidney (LPK) rat. Methods: Complete blood count, systolic blood pressure, urea and creatinine concentration, urinary protein to creatinine ratio, and plasma EPO concentration were determined in control Lewis (n = 51) and LPK rats (n = 56) aged 6-24 weeks. Renal EPO gene expression and RBC osmotic fragility were also documented. Hematopoiesis in spleen and bone marrow were assessed. Results: Lewis polycystic kidney rats had increasing urea and creatinine concentrations, concurrent with the development of a nonregenerative normocytic/normochromic anemia and hypertension, with a significant negative correlation between both HGB and HCT with urea concentration and blood pressure (P < .01). HCT was also significantly negatively correlated with creatinine concentration (P = .014). WBC was significantly negatively correlated with urea (P < .01). Plasma EPO concentration was increased and renal EPO mRNA expression was significantly upregulated in LPK animals. The former was significantly positively correlated with blood pressure and platelet count (P < .05). RBC osmotic fragility was normal in LPK rats and there was no evidence for increased RBC elimination or extramedullary hematopoiesis. Conclusions: Marked anemia in the LPK CKD rodent model in the presence of elevated EPO suggests inefficient erythropoiesis that is correlated with plasma urea concentration and blood pressure.
Details
- Title
- Progression of anemia and its relationship with renal function, blood pressure, and erythropoietin in rats with chronic kidney disease
- Authors/Creators
- J.K. Phillips (Author/Creator)R. Boyd (Author/Creator)M.B. Krockenberger (Author/Creator)G. Burgio (Author/Creator)
- Publication Details
- Veterinary Clinical Pathology, Vol.44(3), pp.342-354
- Publisher
- American Society for Veterinary Clinical Pathology
- Identifiers
- 991005543062207891
- Copyright
- © 2015 American Society for Veterinary Clinical Pathology
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.1030 Erythropoietin Therapy
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science