Journal article
Properties of nucleic acid staining dyes used in gel electrophoresis
Electrophoresis, Vol.36(6), pp.941-944
2015
Abstract
Nucleic acid staining dyes are used for detecting nucleic acids in electrophoresis gels. Historically, the most common dye used for gel staining is ethidium bromide, however due to its toxicity and mutagenicity other dyes that are safer to the user and the environment are preferred. This Short Communication details the properties of dyes now available and their sensitivity for detection of DNA and their ability to permeate the cell membrane. It was found that GelRed™ was the most sensitive and safest dye to use with UV light excitation, and both GelGreen™ and Diamond™ Nucleic Acid Dye were sensitive and the safer dyes using blue light excitation.
Details
- Title
- Properties of nucleic acid staining dyes used in gel electrophoresis
- Authors/Creators
- A.M. Haines (Author/Creator) - Flinders UniversityS.S. Tobe (Author/Creator) - Flinders UniversityH.J. Kobus (Author/Creator) - Flinders UniversityA. Linacre (Author/Creator) - Flinders University
- Publication Details
- Electrophoresis, Vol.36(6), pp.941-944
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005540703607891
- Copyright
- © 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- 1 Clinical & Life Sciences
- 1.189 Genome Studies
- 1.189.310 Population Genetics
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry