Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis

Y-F Wang; X-F Sun; Z-L Han; L. Li; W. Ge; Y. Zhao; M. De Felici; W. Shen; S-F Cheng

doi:10.18632/aging.101405

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Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis

Journal article

Open access

Peer reviewed

Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis

Y-F Wang, X-F Sun, Z-L Han, L. Li, W. Ge, Y. Zhao, M. De Felici, W. Shen and S-F Cheng

Aging, Vol.10(3), pp.463-480

2018

DOI: https://doi.org/10.18632/aging.101405

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Abstract

In this paper, we show that neonatal mice injected for five consecutive days with nicotine display impaired germ cell cyst breakdown and primordial follicle assembly resulting in decreased ovarian reserve lasting until sex maturation age. The effects of nicotine on the pups ovaries were associated with decreased expression of oocyte specific genes such as Nobox, Lhx8, Figlα and Sohlh2. Moreover, the ovaries of pups injected with nicotine showed increased level of cell oxidative stress and autophagic markers (upregulation of AMPKα-1, increased ratio LC3-II/LC3-I, downregulation of AKT and mTOR). Noteworthy, all these effects were counteracted by the administration of the hormone melatonin in 1 μM. In vitro culture of 0 dpp ovaries for 5 days in the presence of 10 μM nicotine reproduced its effect on germ cell cyst breakdown and primordial follicle assembly, furthermore it also revealing about 20% reduction of somatic cells proliferation, and these effects was prevented when melatonin was added to the medium. Taken together these results show that nicotine exposure can adversely affect the establishment of the ovarian reserve in the mouse likely by locally inducing cellular stress altering the primordial follicle assembly and that melatonin, however, is able to counteract such effects.

Details

Title: Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis
Authors/Creators: Y-F Wang (Author/Creator) - Qingdao Agricultural University
X-F Sun (Author/Creator) - Qingdao Agricultural University
Z-L Han (Author/Creator) - First Affiliated Hospital of Chinese PLA General Hospital
L. Li (Author/Creator) - Qingdao Agricultural University
W. Ge (Author/Creator) - Qingdao Agricultural University
Y. Zhao (Author/Creator) - Qingdao Agricultural University
M. De Felici (Author/Creator) - University of Rome Tor Vergata
W. Shen (Author/Creator) - Qingdao Agricultural University
S-F Cheng (Author/Creator) - Qingdao Agricultural University
Publication Details: Aging, Vol.10(3), pp.463-480
Publisher: Impact Journals
Identifiers: 991005540543707891
Copyright: © 2018 Wang et al.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.1408 Fertility Preservation
Web Of Science research areas: Cell Biology; Geriatrics & Gerontology
ESI research areas: Molecular Biology & Genetics